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Literature summary for 3.4.22.B5 extracted from

  • Judice, W.A.; Mottram, J.C.; Coombs, G.H.; Juliano, M.A.; Juliano, L.
    Specific negative charges in cysteine protease isoforms of Leishmania mexicana are highly influential on the substrate binding and hydrolysis (2005), Mol. Biochem. Parasitol., 144, 36-43.
    View publication on PubMed
Search for more literature for 3.4.22.B5

Activating Compound

Activating Compound Comment Organism Structure
NaCl activation of rCPB2.8 and rCPB3 in acidic pH Leishmania mexicana

Application

Application Comment Organism
medicine CPB isoenzymes with a few negative charge modifications provide the parasite with an array of hydrolytic activity and enzymatic adaptation to pH, salinity, and temperature that may be needed for its interaction with the mammalian host Leishmania mexicana

Protein Variants

Protein Variants Comment Organism
H84Y mutation on the outer loop residue (His84 to Tyr) Leishmania mexicana

Inhibitors

Inhibitors Comment Organism Structure
NaCl inhibition of rCPB2.8 and rCPB3 at pH higher than 5 Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Leishmania mexicana ?
-
 ?
Abz-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Leishmania mexicana ?
-
 ?
Z-FR-7-amido-4-methylcoumarin + H2O
-
 Leishmania mexicana ?
-
 ?

Synonyms

Synonyms Comment Organism
CPB2.8
-
 Leishmania mexicana
CPB3
-
 Leishmania mexicana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 50
-
 Leishmania mexicana

pH Range

pH Minimum pH Maximum Comment Organism
3 11
-
 Leishmania mexicana