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Literature summary for 3.4.22.B20 extracted from

  • Halio, S.B.; Bauer, M.W.; Mukund, S.; Adams, M.W.W.; Kelly, R.M.
    Purification and characterization of two functional forms of intracellular protease PfpI from the hyperthermophilic archaeon Pyrococcus furiosus (1997), Appl. Environ. Microbiol., 63, 289-295.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B20

Activating Compound

Activating Compound Comment Organism Structure
N-lauryl sarcosine 0.01% v/v, increases proteolytic activity by about 50% Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Pyrococcus furiosus 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 100 mM, increases proteolytic activity by about 50% Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18800
-
 3 * 18800, enzyme form PfpI-C2, SDS-PAGE Pyrococcus furiosus
18800
-
 6 * 18800, enzyme form PfpI-C1, SDS-PAGE Pyrococcus furiosus
59000
-
 PfpI-C2, gel filtration Pyrococcus furiosus
124000
-
 PfpI-C1, gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51732
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of two enzyme forms: PfpI-C1 and PfpI-C2 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
Ala-Phe-Lys-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
Leu-Tyr-7-amido-4-methylcoumarin
-
 Pyrococcus furiosus Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 18800, enzyme form PfpI-C1, SDS-PAGE Pyrococcus furiosus
trimer 3 * 18800, enzyme form PfpI-C2, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
84
-
 enzyme form PfpI-C1, reaction with Leu-Tyr-7-amido-4-methylcoumarinamide Pyrococcus furiosus
86
-
 enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus
97
-
 enzyme form PfpI-C1, reaction with Ala-Phe-Lys-7-amido-4-methylcoumarinamide Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 110 50°C: about 55% of maximal activity, 110°C: about 45% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half life is 45 min, in presence of 0.5 M KHPO4 the half-life is extended to 80 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus
95
-
 5 min, no inactivation Pyrococcus furiosus
98
-
 half life is below 30 min, in presence of 0.8 M KHPO4 the half-life is extended to 110 min, activity with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.5 pH 5.2: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, enzyme form PfpI-C1, reaction with Ala-Ala-Phe-7-amido-4-methylcoumarinamide Pyrococcus furiosus

pI Value

Organism Comment pI Value Maximum pI Value
Pyrococcus furiosus enzyme form PfpI-C1
-
 6.1