BRENDA - Enzyme Database
show all sequences of 3.4.22.B14

Coronavirus papain-like endopeptidases

Kanjanahaluethai, A.; Baker, S.C.; Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press 2, 1265-1269 (2004)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Murine hepatitis virus
Inhibitors
Inhibitors
Commentary
Organism
Structure
E-64d
membrane-permeable do the irreversible cysteine proteinase inhibitor E-64 blocks cleavage of p65 in vivo
Murine hepatitis virus
leupeptin
blocks the ability of PLP1 to cleave p28 in vitro but not in vivo due to permeability and toxicity issues
Murine hepatitis virus
ZnCl2
blocks the ability of PLP1 to cleave p28 in vitro but not in vivo due to permeability and toxicity issues
Murine hepatitis virus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
-
Murine hepatitis virus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
p28
Murine hepatitis virus
-
?
-
-
?
p65
Murine hepatitis virus
-
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Murine hepatitis virus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Murine hepatitis virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
p28
-
668774
Murine hepatitis virus
?
-
-
-
?
p65
-
668774
Murine hepatitis virus
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
first papain-like cysteine proteinase
-
Murine hepatitis virus
PLP1
-
Murine hepatitis virus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Murine hepatitis virus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
E-64d
membrane-permeable do the irreversible cysteine proteinase inhibitor E-64 blocks cleavage of p65 in vivo
Murine hepatitis virus
leupeptin
blocks the ability of PLP1 to cleave p28 in vitro but not in vivo due to permeability and toxicity issues
Murine hepatitis virus
ZnCl2
blocks the ability of PLP1 to cleave p28 in vitro but not in vivo due to permeability and toxicity issues
Murine hepatitis virus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
-
Murine hepatitis virus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
p28
Murine hepatitis virus
-
?
-
-
?
p65
Murine hepatitis virus
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Murine hepatitis virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
p28
-
668774
Murine hepatitis virus
?
-
-
-
?
p65
-
668774
Murine hepatitis virus
?
-
-
-
?
Other publictions for EC 3.4.22.B14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755215
Niemeyer
The papain-like protease dete ...
SARS coronavirus FRA
PLoS Pathog.
14
e1007296
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
752911
Hu
Transmissible gastroenteritis ...
Transmissible gastroenteritis virus
BioMed Res. Int.
2017
7089091
2017
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
754425
Daczkowski
Structural insights into the ...
SARS coronavirus
J. Mol. Biol.
429
1661-1683
2017
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755428
Li
SARS coronavirus papain-like ...
SARS coronavirus
Sci. Rep.
6
25754
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
752708
Bekes
SARS hCoV papain-like proteas ...
Middle East respiratory syndrome-related coronavirus, SARS coronavirus
Biochem. J.
468
215-226
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
754125
Kong
Structural view and substrate ...
infectious bronchitis virus, infectious bronchitis virus Beaudette
J. Biol. Chem.
290
7160-7168
2015
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
709746
Wojdyla
Papain-like protease 1 from tr ...
Transmissible gastroenteritis virus, Transmissible gastroenteritis virus Purdue
J. Virol.
84
10063-10073
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
8
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
709752
Gadlage
Exchange of the coronavirus re ...
Murine hepatitis virus
J. Virol.
84
6894-6898
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
709449
Ghosh
Structure-based design, synthe ...
SARS coronavirus
J. Med. Chem.
52
5228-5240
2009
-
-
1
-
-
-
22
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
21
-
-
1
-
-
-
-
21
22
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670849
Sulea
Binding site-based classificat ...
bovine coronavirus, bovine coronavirus Ent, Human coronavirus 229E, infectious bronchitis virus, infectious bronchitis virus Beaudette, Murine hepatitis virus, Murine hepatitis virus A59, porcine epidemic diarrhea virus, SARS coronavirus, Transmissible gastroenteritis virus
Proteins Struct. Funct. Bioinform.
62
760-775
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681672
Graham
Replication of murine hepatiti ...
Murine hepatitis virus
J. Virol.
80
11610-11620
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668774
Kanjanahaluethai
-
Coronavirus papain-like endope ...
Murine hepatitis virus
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press
2
1265-1269
2004
-
-
1
-
-
-
3
-
-
-
1
2
-
1
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670071
Harcourt
Identification of severe acute ...
SARS coronavirus, SARS coronavirus Urbani
J. Virol.
78
13600-13612
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
647411
Teng
Further in vitro characterizat ...
Murine coronavirus, Murine coronavirus JHM
J. Neurovirol.
8
143-149
2002
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
647410
Teng
Expression of murine coronavir ...
Murine coronavirus, Murine coronavirus JHM
J. Virol.
73
2658-2666
1999
-
-
1
-
-
-
-
-
-
-
-
2
-
7
-
-
1
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
647412
Bonilla
Characterization of the leader ...
Murine coronavirus, Murine coronavirus JHM
Virology
209
489-497
1995
-
-
1
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-