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Literature summary for 3.4.22.8 extracted from
- Clostripain (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 699-719.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Borate | partial inhibition | Hathewaya histolytica |  |
| butylguanidine | most effective competitive inhibitor, hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
| Cd2+ | - |
Hathewaya histolytica | |
| citrate | partial inhibition | Hathewaya histolytica | |
| Co2+ | - |
Hathewaya histolytica | |
| Cu2+ | - |
Hathewaya histolytica | |
| EDTA | - |
Hathewaya histolytica | |
| H2O2 | complete regeneration by reduction with dithiothreitol | Hathewaya histolytica | |
| K+ | - |
Hathewaya histolytica | |
| Na+ | - |
Hathewaya histolytica | |
| PCMB | - |
Hathewaya histolytica | |
| Polyarginine | hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
| polylysine | hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
| RCM RNase | hydrolysis of benzoyl-Arg ethyl ester | Hathewaya histolytica | |
| Tris | partial inhibition | Hathewaya histolytica | |
| Veronal | partial inhibition | Hathewaya histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
p-toluenesulfonyl-L-Arg methyl ester | - |
Hathewaya histolytica | |
| 0.22 | - |
benzoyl-Arg ethyl ester | - |
Hathewaya histolytica | |
| 0.25 | - |
Thr-Ala-Ala | - |
Hathewaya histolytica | |
| 1.3 | - |
N-benzoyl-L-Arg amide | - |
Hathewaya histolytica | |
| 2.5 | - |
p-Nitrophenyl p-guanidinobenzoate | - |
Hathewaya histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances esterase activity | Hathewaya histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
- |
Hathewaya histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Arg methyl ester + H2O | - |
Hathewaya histolytica | L-Arg + methanol | - |
? | |
| additional information | the major activity is directed towards the carboxyl peptide linkage of Arg | Hathewaya histolytica | ? | - |
? | |
| N-benzoyl-Arg amide + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + NH3 | - |
? | |
| N-benzoyl-Arg benzyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + benzyl alcohol | - |
? | |
| N-benzoyl-Arg ethyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + ethanol | - |
? | |
| N-benzoyl-Arg isopropyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg isopropanol | - |
? | |
| N-benzoyl-Arg methyl ester + H2O | - |
Hathewaya histolytica | N-benzoyl-Arg + methanol | - |
? | |
| Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O | - |
Hathewaya histolytica | Nalpha-benzoyl-L-Arg + 2-naphthylamine | - |
? | |
| p-nitrophenyl p-guanidinobenzoate + H2O | - |
Hathewaya histolytica | p-nitrophenol + p-guanidinobenzoate | - |
? | |
| p-tosyl-Arg methyl ester + H2O | - |
Hathewaya histolytica | p-tosyl-Arg + methanol | - |
? | |
| Thr-Ala-Ala + H2O | - |
Hathewaya histolytica | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ca2+ depresses thermal inactivation | Hathewaya histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
hydrolysis of L-Arg methyl ester, in phosphate buffer | Hathewaya histolytica |
| 7.4 | 7.8 | hydrolysis of alpha-benzoyl-Arg ethyl ester | Hathewaya histolytica |
| 7.4 | 7.8 | in phosphate or Tris-HCl buffer | Hathewaya histolytica |
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