Literature summary for 3.4.22.8 extracted from

Kim, C.K.; Lee, S.Y.; Kwon, O.J.; Lee, S.M.; Nah, S.Y.; Jeong, S.M.
Secretory expression of active clostripain in Escherichia coli (2007), J. Biotechnol., 131, 346-352.

Application

Application	Comment	Organism
synthesis	recombinant clostripain might prove useful in the production of insulin from the proinsulin fusion protein	Clostridium sp.

Cloned(Commentary)

Cloned (Comment)	Organism
clostripain gene is modified and its signal sequence is replaced with that of penicillin G acylase. The core clostripain protein fused to the penicillin G acylase signal peptide is also prepared. With regard to the expression of the clostripain precursors, the majority of clostripain activity is observed in the culture media, thereby indicating that both the clostripain signal peptide and the penicillin G acylase signal peptide are recognized in the Escherichia coli secretion pathway, and the precursors successfully mature into the active form. The activity is rather low when the core protein is expressed, which indicates that the clostripain pro-peptide is important in the formation of the active enzyme in Escherichia coli	Clostridium sp.

Cloned (Comment)

Organism

clostripain gene is modified and its signal sequence is replaced with that of penicillin G acylase. The core clostripain protein fused to the penicillin G acylase signal peptide is also prepared. With regard to the expression of the clostripain precursors, the majority of clostripain activity is observed in the culture media, thereby indicating that both the clostripain signal peptide and the penicillin G acylase signal peptide are recognized in the Escherichia coli secretion pathway, and the precursors successfully mature into the active form. The activity is rather low when the core protein is expressed, which indicates that the clostripain pro-peptide is important in the formation of the active enzyme in Escherichia coli

Clostridium sp.

Organism

Organism	UniProt	Comment	Textmining
Clostridium sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
proinsulin fusion protein + H2O	clostripain specifically cleaves the arginine residues in the leader peptide and in the C-peptide	Clostridium sp.	insulin + ?	-	?

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Release 2023.1 (January 2023)