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Literature summary for 3.4.22.71 extracted from

  • Kang, H.J.; Coulibaly, F.; Proft, T.; Baker, E.N.
    Crystal structure of Spy0129, a Streptococcus pyogenes class B sortase involved in pilus assembly (2011), PLoS ONE, 6, e15969.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.71

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pRP cells Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging or sitting drop vapor diffusion method, using 10% (w/v) polyethylene glycol 3350, 0.2 M Zn(OAc)2, 0.1 M NaOAc, pH 6.2 Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
C221S the single residue mutation does not have noticeable effect on the secondary structure of the enzyme Streptococcus pyogenes

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the asymmetric unit contains 19 bound Zn2+ ions Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes M1 SF370
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-charged HiTrap column chromatography and Superdex 200 gel filtration Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-KDFEVPTGVAM-Dap(Dnp)-NH2 + H2O
-
 Streptococcus pyogenes ?
-
 ?
Abz-KDFEVPTGVAM-Dap(Dnp)-NH2 + H2O
-
 Streptococcus pyogenes M1 SF370 ?
-
 ?

Synonyms

Synonyms Comment Organism
class B sortase
-
 Streptococcus pyogenes
Spy0129
-
 Streptococcus pyogenes

General Information

General Information Comment Organism
physiological function the class B sortase is involved in pilus assembly Streptococcus pyogenes