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Literature summary for 3.4.22.70 extracted from

  • Jia, X.; Kwon, S.; Wang, C.I.; Huang, Y.H.; Chan, L.Y.; Tan, C.C.; Rosengren, K.J.; Mulvenna, J.P.; Schroeder, C.I.; Craik, D.J.
    Semienzymatic cyclization of disulfide-rich peptides using sortase A (2014), J. Biol. Chem., 289, 6627-6638.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.70

Application

Application Comment Organism
synthesis semienzymatic cyclization of disulfide-rich peptides using sortase A, overview Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
gene srtA, recombinant expression of His6-tagged enzyme catalytic domain, comprising residues 60-206, in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Staphylococcus aureus the enzyme recognizes a 5-residue sequence motif, LPXTG, and cleaves the peptide bond between the Thr and Gly residues, forming an acyl enzyme intermediate between a cysteine at the active site of SrtA and the carboxylate at the truncated C-terminus of the substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme catalytic domain from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatograph Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme recognizes a 5-residue sequence motif, LPXTG, and cleaves the peptide bond between the Thr and Gly residues, forming an acyl enzyme intermediate between a cysteine at the active site of SrtA and the carboxylate at the truncated C-terminus of the substrate Staphylococcus aureus ?
-
 ?
additional information semienzymatic cyclization of disulfide-rich peptides using sortase A, including the cyclotide kalata B1, alpha-conotoxin Vc1.1, and sunflower trypsin inhibitor 1, without the need for a thioester linker and using Fmoc chemistry, NMR spectral structure analysis, overview Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
SrtA
-
 Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Staphylococcus aureus