Any feedback?
Literature summary for 3.4.22.70 extracted from
- Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A (2008), Biotechnol. Lett., 30, 1603-1607.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A. This method provides a robust and gentle approach for the site-directed, covalent coupling of proteins to biosensor chips. The high specificity of the sortase allows immobilization of proteins from less than pure protein samples allowing short cuts in protein purification protocols | Staphylococcus aureus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant mature sortase A (without the N-terminal signal peptide) is produced as GST fusion in Escherichia coli | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant sortase A-GST fusion protein | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SrtA | - |
Staphylococcus aureus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
