Literature summary for 3.4.22.70 extracted from

Bentley, M.L.; Gaweska, H.; Kielec, J.M.; McCafferty, D.G.
Engineering the substrate specificity of Staphylococcus aureus sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA (2007), J. Biol. Chem., 282, 6571-6581.

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Cloned(Commentary)

Cloned (Comment)	Organism
N-terminally His6-tagged SrtADELTAN24 is expressed in Escherichia coli BL21	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
additional information	marked changes in the specificity profile of SrtA are obtained by replacing the beta6/beta7 loop in SrtA with the corresponding domain from SrtB. The chimeric beta6/beta7 loop swap enzyme (SrtLS) conferrs the ability to acylate NPQTN-containing substrates, with a kcat/Kmapp of 0.0062 /M * s. This enzyme is unable to perform the transpeptidation stage of the reaction, suggesting that additional domains are required for transpeptidation to occur. The overall catalytic specificity profile (kcat/Kmapp (NPQTN)/kcat/Kmapp (LPETG)) of SrtLS is altered 700000fold from SrtA. These results indicate that the beta6/beta7 loop is an important site for substrate recognition in sortases	Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
N-terminally His6-tagged SrtADELTAN24 expressed in Escherichia coli BL21	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-KVENPQTNAGT-Dap(Dnp)-NH2 + GGGGG	-	Staphylococcus aureus	Abz-KVENPQTGGGGG + Gly-Dap(DNP)-NH2	-	?

Synonyms

Synonyms	Comment	Organism
SrtA	-	Staphylococcus aureus

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Release 2023.1 (January 2023)