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Literature summary for 3.4.22.70 extracted from

  • Parthasarathy, R.; Subramanian, S.; Boder, E.T.
    Sortase A as a novel molecular "stapler" for sequence-specific protein conjugation (2007), Bioconjug. Chem., 18, 469-476.
    View publication on PubMed

Application

Application Comment Organism
synthesis in vitro applications of sortase A to protein conjugation. Application of recombinant Staphylococcus aureus sortase A to attach a tagged model protein substrate (green fluorescent protein) to polystyrene beads chemically modified with either alkylamine or the in vivo sortase A ligand, Gly-Gly-Gly, on their surfaces. Sortase A can be used to sequence-specifically ligate eGFP to amino-terminated poly(ethylene glycol) and to generate protein oligomers and cyclized monomers using suitably tagged eGFP An alkylamine can substitute for the natural Gly3 substrate, which suggests the possibility of using the enzyme in materials applications. The highly specific and mild sortase A-catalyzed reaction, based on small recognition tags unlikely to interfere with protein expression represents a useful addition to the protein immobilization and modification tool kit Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
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