Literature summary for 3.4.22.70 extracted from

Aulabaugh, A.; Ding, W.; Kapoor, B.; Tabei, K.; Alksne, L.; Dushin, R.; Zatz, T.; Ellestad, G.; Huang, X.
Development of an HPLC assay for Staphylococcus aureus sortase: evidence for the formation of the kinetically competent acyl enzyme intermediate (2007), Anal. Biochem., 360, 14-22.

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Application

Application	Comment	Organism
analysis	development of a reverse-phase HPLC assay to identify and characterize sortase reaction intermediates	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	Gly-Gly-Gly	-	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aminobenzoyl-LPETG-dinitrophenyl ester + Gly-Gly-Gly	HPLC results provide direct evidence for the formation of the kinetically competent acyl enzyme intermediate	Staphylococcus aureus	aminobenzoyl-LPETGGG + Gly-dinitrophenyl ester	-	?

Synonyms

Synonyms	Comment	Organism
SrtA	-	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.18	-	Gly-Gly-Gly	-	Staphylococcus aureus

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Release 2023.1 (January 2023)