Any feedback?
Literature summary for 3.4.22.70 extracted from
- Crystal structures of Staphylococcus aureus sortase A and its substrate somplex (2004), J. Biol. Chem., 279, 31383-31389.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals are grown by the hanging-drop technique with a protein concentration of 50 mg/ml in 25 mM MES buffer, pH 6.35. The crystallization conditions include 3.2 M ammonium sulfate, 0.1 M NaCl, and trace amounts of ethylene glycol. Crystal structure of native SrtA, of an active-site mutant of SrtA, and of the mutant SrtA complexed with its substrate LPETG peptide | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA N59 | trucation of N-terminal 59 amino acids does not affect the activity of the enzyme | Staphylococcus aureus |
| DELTA N59 | enhanced solubility of the mutant helps the crystallization | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
