Literature summary for 3.4.22.70 extracted from

Scott, C.J.; McDowell, A.; Martin, S.L.; Lynas, J.F.; Vandenbroeck, K.; Walker, B.
Irreversible inhibition of the bacterial cysteine protease-transpeptidase sortase (SrtA) by substrate-derived affinity labels (2002), Biochem. J., 366, 953-958.

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Application

Application	Comment	Organism
medicine	substrate-derived irreversible inhibitors of SrtA that might find application in delineating the role of the cysteine protease-transpeptidase in cell surface protein sorting and adherence of Gram-positive organisms	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
benzyloxycarbonyl-Leu-Pro-Ala-Thr-CH2Cl	irreversible inhibitor of recombinant enzyme	Staphylococcus aureus
benzyloxycarbonyl-Leu-Pro-Ala-Thr-CHN2	irreversible inhibitor of recombinant enzyme	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Gln-Ala-Leu-Pro-Glu-Thr-Gly-Glu-Glu-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O	-	Staphylococcus aureus	4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Gln-Ala-Leu-Pro-Glu-Thr + Gly-Glu-Glu-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00000021	-	benzyloxycarbonyl-Leu-Pro-Ala-Thr-CH2Cl	pH 7.5, 37°C	Staphylococcus aureus
0.00000022	-	benzyloxycarbonyl-Leu-Pro-Ala-Thr-CHN2	pH 7.5, 37°C	Staphylococcus aureus

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Release 2023.1 (January 2023)