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Literature summary for 3.4.22.7 extracted from
- Purification and preliminary characterization of two asclepains from the latex of Asclepias syriaca L. (milkweed) (1979), Biochim. Biophys. Acta, 578, 13-22.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetic acid | - |
Asclepias syriaca |  |
| PCMB | - |
Asclepias syriaca | |
| Sodium tetrathionate | - |
Asclepias syriaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
asclepain B5, gel filtration | Asclepias syriaca |
| 23000 | - |
1 * 23000, carboxymethylated asclepain A3 and carboxymethylated asclepain B5, SDS-PAGE | Asclepias syriaca |
| 24000 | - |
asclepain A3, gel filtration | Asclepias syriaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Asclepias syriaca | - |
asclepain A3 and B5 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | no bound carbohydrate | Asclepias syriaca |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Asclepias syriaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Asclepias syriaca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4100 | - |
asclepain A3 | Asclepias syriaca |
| 6300 | - |
ascepain B5 | Asclepias syriaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-L-Arg-p-nitroanilide + H2O | - |
Asclepias syriaca | benzoyl-L-Arg + 4-nitroaniline | - |
? | |
| casein + H2O | - |
Asclepias syriaca | ? | - |
? | |
| N-carbobenzoxy-Gly p-nitrophenyl ester + H2O | - |
Asclepias syriaca | N-carbobenzoxy-Gly + p-nitrophenol | - |
? | |
| N-carbobenzoxy-Leu p-nitrophenyl ester + H2O | - |
Asclepias syriaca | N-carbobenzoxy-Leu + p-nitrophenol | - |
? | |
| N-carbobenzoxy-Tyr p-nitrophenyl ester + H2O | - |
Asclepias syriaca | N-carbobenzoxy-Tyr + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 23000, carboxymethylated asclepain A3 and carboxymethylated asclepain B5, SDS-PAGE | Asclepias syriaca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | hydrolysis of casein, asclepain B5 | Asclepias syriaca |
| 7.5 | 8.5 | hydrolysis of casein, asclepain A3 | Asclepias syriaca |
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