Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.69 extracted from

  • Luo, W.; Su, X.; Gong, S.; Qin, Y.; Liu, W.; Li, J.; Yu, H.; Xu, Q.
    Anti-SARS coronavirus 3C-like protease effects of Rheum palmatum L. extracts (2009), Biosci. Trends, 3, 124-126.
    View publication on PubMed
Search for more literature for 3.4.22.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
extracts of Rheum palmatum
-
 Severe acute respiratory syndrome-related coronavirus
additional information extracts from Rheum palmatum have a high level of inhibitory activity against 3CL protease with IC50 of 0.01376 mg/ml and an inhibition rate of up to 96% Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and anion-exchange column chromatography Severe acute respiratory syndrome-related coronavirus
ammonium sulfate precipitation, anion-exchange chromatography Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Thr-L-Ser-L-Ala-L-Val-L-Leu-L-Gln-4-nitroanilide + H2O
-
 Severe acute respiratory syndrome-related coronavirus L-Thr-L-Ser-L-Ala-L-Val-L-Leu-L-Gln + 4-nitroaniline
-
 ?
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide + H2O
-
 Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
3CL protease
-
 Severe acute respiratory syndrome-related coronavirus
SARS coronavirus 3C-like protease
-
 Severe acute respiratory syndrome-related coronavirus
SARS-3CL protease
-
 Severe acute respiratory syndrome-related coronavirus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 extracts of Rheum palmatum show IC50 values between 0.014 mg/ml and 0.059 mg/ml Severe acute respiratory syndrome-related coronavirus additional information