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Literature summary for 3.4.22.69 extracted from

  • Cheng, S.C.; Chang, G.G.; Chou, C.Y.
    Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease (2010), Biophys. J., 98, 1327-1336.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.69

Cloned(Commentary)

Cloned (Comment) Organism
E166A mutant protein expressed in Escherichia coli BL21(DE3) Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
E166A involved in connecting the substrate binding site with the dimer interface, dimerization influenced by substrate binding Severe acute respiratory syndrome-related coronavirus
E166A/R298A monomer Severe acute respiratory syndrome-related coronavirus
R298A induce dimer dissociation (influenced by substrate binding), about 10fold decrease in activity Severe acute respiratory syndrome-related coronavirus
R298A/Q299A monomer, no activity detected Severe acute respiratory syndrome-related coronavirus
R298L induce dimer dissociation (influenced by substrate binding), about 10fold decrease in activity Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2226
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide wild-type protein Severe acute respiratory syndrome-related coronavirus
0.3534
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide E166A mutant protein Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
E166A mutant protein: immobilized metal ion affinity chromatography (Ni2+) Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide + H2O
-
 Severe acute respiratory syndrome-related coronavirus Thr-Ser-Ala-Val-Leu-Gln + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
homodimer analytical ultracentrifugation, gel filtration Severe acute respiratory syndrome-related coronavirus

Synonyms

Synonyms Comment Organism
SARS-coronavirus main protease
-
 Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide R298A mutant protein Severe acute respiratory syndrome-related coronavirus
0.31
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide E166A mutant protein Severe acute respiratory syndrome-related coronavirus
0.48
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide R298L mutant protein Severe acute respiratory syndrome-related coronavirus
0.63
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide wild-type protein Severe acute respiratory syndrome-related coronavirus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.877
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide E166A mutant protein Severe acute respiratory syndrome-related coronavirus
2.83
-
 Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide wild-type protein Severe acute respiratory syndrome-related coronavirus