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Literature summary for 3.4.22.68 extracted from

  • Hermkes, R.; Fu, Y.-F.; Nuerrenberg, K.; Budhiraja, R.; Schmelzer, E.; Elrouby, N.; Dohmen, R.J.; Bachmair, A.; Coupland, G.
    Distinct roles for Arabidopsis SUMO protease ESD4 and its closest homolog ELS1 (2011), Planta, 233, 63-73.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.68

Cloned(Commentary)

Cloned (Comment) Organism
gene ESD4, DNA and amino acid sequence determination, genotyping Arabidopsis thaliana
gene ULP1A, DNA and amino acid sequence determination, genotyping, recombinant expression in Arabidopsis thaliana plants and in leaves of Nicotioana bentamiana using transfection via Agrobacterium tumefaciens. An expressed GFP-ELS1 construct has a generally non-nuclear localization, being detectable at membranes and in the cytoplasm Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C461S site-directed mutagenesis, ELS1C461S is not capable of cleaving the extension oV the carboxyl terminus of SUMO1 Arabidopsis thaliana
additional information plant enzymes ELS1 and ESD4 differ in their ability to complement yeast Ulp1 mutants, mutant phenotypes, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Arabidopsis thaliana 5737
-
membrane
-
 Arabidopsis thaliana 16020
-
additional information not in the nucleus Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
small ubiquitin-related modifier-protein + H2O Arabidopsis thaliana SUMO-specific proteases, SENPs, reversibly remove small ubiquitin-related modifier-protein, SUMO, from the SUMOylated proteins small ubiquitin-related modifier-protein + protein
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GYL3 ELS1
-
Arabidopsis thaliana Q94F30 ESD4
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-
silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ELS1, but not ELS1C461S, is capable of cleaving the extension oV the carboxyl terminus of SUMO1 Arabidopsis thaliana ?
-
 ?
small ubiquitin-related modifier-protein + H2O SUMO-specific proteases, SENPs, reversibly remove small ubiquitin-related modifier-protein, SUMO, from the SUMOylated proteins Arabidopsis thaliana small ubiquitin-related modifier-protein + protein
-
 r

Synonyms

Synonyms Comment Organism
ELS1 an ESD4 homologue Arabidopsis thaliana
ESD4
-
 Arabidopsis thaliana
SUMO protease
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction growth defects caused by loss of ESD4 function are not due to increased synthesis of the stress signal salicylic acid, enzyme depletion causes delay in flowering Arabidopsis thaliana
malfunction growth defects caused by loss of ESD4 function are not due to increased synthesis of the stress signal salicylic acid. ELS1 depletion causes no measurable alterations in flowering and leaf development Arabidopsis thaliana
additional information active site residue is Cys461 Arabidopsis thaliana
physiological function SUMO protease ESD4 and ESD4-like SUMO protease 1, i.e. ELS1 or AtULP1a, show close sequence similarity, but different properties and are functionally distinct, overview Arabidopsis thaliana