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Literature summary for 3.4.22.67 extracted from
- Three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes (2015), Int. J. Biol. Macromol., 73, 245-252.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | strong inhibition | Zingiber officinale |  |
| Co2+ | slight inhibition | Zingiber officinale | |
| Cu2+ | complete inhibition | Zingiber officinale | |
| Hg2+ | complete inhibition | Zingiber officinale | |
| Mn2+ | slight inhibition | Zingiber officinale | |
| Zn2+ | slight inhibition | Zingiber officinale | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Zingiber officinale | |
| K+ | activates | Zingiber officinale | |
| additional information | the purified enzyme is highly stable against numerous metal ions | Zingiber officinale | |
| Na+ | activates | Zingiber officinale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33800 | - |
native PAGE | Zingiber officinale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zingiber officinale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 14.91fold from rhizomes by ammonium sulfate fractionation, tert-butanol precipitation at 25°C and pH 7.0 using the interfacial precipitateand the aqueous phase, and ultrafiltration, method optimization | Zingiber officinale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| rhizome | - |
Zingiber officinale | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.005 | - |
purified native enzyme, substrate bovine casein, pH 7.0, 40°C | Zingiber officinale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bovine casein + H2O | - |
Zingiber officinale | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33800, SDS-PAGE | Zingiber officinale |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| zingibain | - |
Zingiber officinale |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Zingiber officinale |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | purified native enzyme, pH 7.0, 2 h, stable | Zingiber officinale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
broad optimum at pH 6.0-7.5 | Zingiber officinale |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | activity range, profile overview | Zingiber officinale |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Zingiber officinale | isoelectric focussing | - |
4.38 |
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Release 2023.1 (January 2023)
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