BRENDA - Enzyme Database show
show all sequences of 3.4.22.66

Insights into the enzyme-substrate interaction in the norovirus 3C-like protease

Someya, Y.; Takeda, N.; J. Biochem. 146, 509-521 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells
Chiba virus
Engineering
Amino acid exchange
Commentary
Organism
E54L
the E54L mutant protease is a substrate-specificity mutant and requires large hydrophobic amino acid residues at both P4 and P2 positions of the substrate, it cleaves the sequence 133LSFE/AP between the 3B and 3C regions of norovirus polyprotein, but does not cleaves the sequence 198ATSE/GK between the 3A and 3B
Chiba virus
Inhibitors
Inhibitors
Commentary
Organism
Structure
L-Leu-Gly-Gly
-
Chiba virus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
norovirus polyprotein + H2O
Chiba virus
-
hydrolyzed norovirus polyprotein
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chiba virus
-
-
-
Purification (Commentary)
Commentary
Organism
GSTrap FF column chromatography
Chiba virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
norovirus polyprotein + H2O
-
708950
Chiba virus
hydrolyzed norovirus polyprotein
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells
Chiba virus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E54L
the E54L mutant protease is a substrate-specificity mutant and requires large hydrophobic amino acid residues at both P4 and P2 positions of the substrate, it cleaves the sequence 133LSFE/AP between the 3B and 3C regions of norovirus polyprotein, but does not cleaves the sequence 198ATSE/GK between the 3A and 3B
Chiba virus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-Leu-Gly-Gly
-
Chiba virus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
norovirus polyprotein + H2O
Chiba virus
-
hydrolyzed norovirus polyprotein
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
GSTrap FF column chromatography
Chiba virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
norovirus polyprotein + H2O
-
708950
Chiba virus
hydrolyzed norovirus polyprotein
-
-
-
?
Other publictions for EC 3.4.22.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732410
Kim
Broad-spectrum antivirals agai ...
Foot-and-mouth disease virus, Hepatovirus A, Human calicivirus Hu/NLV/GII/MD145-12/1987/US, Norwalk virus, SARS coronavirus, Transmissible gastroenteritis virus, Transmissible gastroenteritis virus Purdue
J. Virol.
86
11754-11762
2012
-
6
-
2
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-
30
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-
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-
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13
-
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7
-
6
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6
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24
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6
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2
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24
30
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-
-
-
-
7
-
6
-
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-
6
-
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-
-
-
-
-
-
-
718084
Oka
Comparative site-directed muta ...
feline calicivirus, Norwalk virus, Rabbit hemorrhagic disease virus, Sapporo virus
Microbiol. Immunol.
55
108-114
2011
-
-
-
-
8
-
-
-
-
-
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11
-
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8
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8
8
-
-
-
693255
Lee
Structural basis of inhibition ...
Human coronavirus 229E
J. Biol. Chem.
284
7646-7655
2009
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1
1
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5
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6
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1
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1
1
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1
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1
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5
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1
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1
1
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-
-
-
-
-
-
1
1
-
-
-
708950
Someya
Insights into the enzyme-subst ...
Chiba virus
J. Biochem.
146
509-521
2009
-
-
1
-
1
-
1
-
-
-
-
1
-
2
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
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-
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-
-
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-
688730
Oka
Highly conserved configuration ...
feline calicivirus, Sapovirus
J. Virol.
81
6798-6806
2007
-
-
2
-
-
-
-
-
-
-
-
-
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6
-
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2
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2
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
670097
Zeitler
X-ray crystallographic structu ...
Norwalk virus
J. Virol.
80
5050-5058
2006
1
1
1
1
1
-
2
-
-
-
-
-
-
4
-
-
1
-
-
-
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1
1
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1
1
1
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1
1
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2
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1
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1
1
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-
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670711
Asanaka
-
Replication and packaging of N ...
Norwalk virus
Proc. Natl. Acad. Sci. USA
19
10327-32
2006
-
1
1
-
-
-
-
-
-
-
-
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1
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1
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1
1
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-
1
-
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-
-
-
-
-
-
-
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-
-
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688725
Sosnovtsev
Cleavage map and proteolytic p ...
Murine norovirus
J. Virol.
80
7816-7831
2006
-
-
-
-
-
-
-
-
-
-
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-
-
4
-
-
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1
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1
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-
670082
Nakamura
A norovirus protease structure ...
Chiba virus
J. Virol.
79
13685-13693
2005
-
1
1
1
-
-
-
-
-
-
-
-
-
3
-
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1
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1
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1
1
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
670086
Belliot
Norovirus proteinase-polymeras ...
norovirus, norovirus MD145
J. Virol.
79
2393-2403
2005
-
1
-
-
-
-
-
-
-
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2
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2
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2
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-
671000
Someya
Characterization of the norovi ...
Chiba virus
Virus Res.
110
91-97
2005
1
-
1
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1
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12
-
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1
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4
-
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1
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1
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1
1
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1
1
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1
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1
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1
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12
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1
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1
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1
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1
1
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1
1
-
-
-
-
-
-
-
-
670075
Kuyumcu-Martinez
Calicivirus 3C-like proteinase ...
Enterovirus C, feline calicivirus, norovirus, norovirus MD145-12
J. Virol.
78
8172-8182
2004
-
2
2
-
-
-
-
-
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11
-
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4
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2
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4
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-
647402
Liu
Identification of further prot ...
Southampton virus
J. Gen. Virol.
80
291-296
1999
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1
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1
1
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4
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1
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1
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647401
Liu
Polyprotein processing in Sout ...
Southampton virus
J. Virol.
70
2605-2610
1996
-
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1
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-
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5
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647400
Lambden
A conserved sequence motif at ...
Southampton virus
Virus Genes
10
149-152
1995
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647399
Lambden
Sequence and genome organizati ...
Southampton virus
Science
259
516-519
1993
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