Application | Comment | Organism |
---|---|---|
diagnostics | production of recombinant enzymatically and immunologically enzyme for diagnostic testing | Dermatophagoides farinae |
medicine | production of recombinant enzyme for allergen-specific immunotherapy | Dermatophagoides farinae |
pharmacology | production of recombinant enzymatically and immunologically enzyme for deciphering of immunotherapy mechanisms | Dermatophagoides farinae |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of the cDNA sequence encoding proenzyme pro-Der f1, recombinant expression od Strep-tagged proenzyme in Escherichia coli in inclusion bodies from vector pET-44a | Dermatophagoides farinae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dermatophagoides farinae | P16311 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant proenzyme pro-Der f1 from Escherichia coli after solubilization from inclusion bodies and refolding by dialysis and affinity chromatography | Dermatophagoides farinae |
Renatured (Comment) | Organism |
---|---|
recombinant proenzyme pro-Der f1 from Escherichia coli inclusion bodies, solubilization/degenration by 6 M urea, and refolding in refolding buffer containing 20 mM Tris.Cl pH 8.6, 1% glycerol, 1 mM EDTA, as well as 3 mM reduced glutathione, and 0.3 mM oxidized glutathione at a final concentration of 0.05 mg/ml, followed by gel filtration, method optimization, overview | Dermatophagoides farinae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Dermatophagoides farinae | N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mite allergen | - |
Dermatophagoides farinae |
pro-Der f1 | - |
Dermatophagoides farinae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Dermatophagoides farinae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Dermatophagoides farinae |