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Literature summary for 3.4.22.65 extracted from

  • Ling, C.; Zhang, J.; Chen, H.; Zou, Z.; Lai, H.; Zhang, J.; Lin, D.; Tao, A.
    Expression and refolding of mite allergen pro-Der f1 from inclusion bodies in Escherichia coli (2015), Protein Expr. Purif., 109, 93-98.
    View publication on PubMed

Application

Application Comment Organism
diagnostics production of recombinant enzymatically and immunologically enzyme for diagnostic testing Dermatophagoides farinae
medicine production of recombinant enzyme for allergen-specific immunotherapy Dermatophagoides farinae
pharmacology production of recombinant enzymatically and immunologically enzyme for deciphering of immunotherapy mechanisms Dermatophagoides farinae

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of the cDNA sequence encoding proenzyme pro-Der f1, recombinant expression od Strep-tagged proenzyme in Escherichia coli in inclusion bodies from vector pET-44a Dermatophagoides farinae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Dermatophagoides farinae P16311
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proenzyme pro-Der f1 from Escherichia coli after solubilization from inclusion bodies and refolding by dialysis and affinity chromatography Dermatophagoides farinae

Renatured (Commentary)

Renatured (Comment) Organism
recombinant proenzyme pro-Der f1 from Escherichia coli inclusion bodies, solubilization/degenration by 6 M urea, and refolding in refolding buffer containing 20 mM Tris.Cl pH 8.6, 1% glycerol, 1 mM EDTA, as well as 3 mM reduced glutathione, and 0.3 mM oxidized glutathione at a final concentration of 0.05 mg/ml, followed by gel filtration, method optimization, overview Dermatophagoides farinae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
Dermatophagoides farinae N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
mite allergen
-
Dermatophagoides farinae
pro-Der f1
-
Dermatophagoides farinae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Dermatophagoides farinae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Dermatophagoides farinae