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Literature summary for 3.4.22.65 extracted from

  • Zhang, J.; Saint-Remy, J.M.; Garrod, D.R.; Robinson, C.
    Comparative enzymology of native and recombinant house dust mite allergen Der p 1 (2009), Allergy, 64, 469-477.
    View publication on PubMed
Search for more literature for 3.4.22.65

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Dermatophagoides pteronyssinus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Dermatophagoides pteronyssinus

Inhibitors

Inhibitors Comment Organism Structure
Der p 1 allergen ADZ 50,000, irreversible inactivation, active site titrant Dermatophagoides pteronyssinus
Der p 1 prodomain PP-02, recombinant, apparently competitive Dermatophagoides pteronyssinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0154
-
 (2-aminobenzoyl)-Val-Ala-norLeu-Ser-Tyr(3-NO2)-Asp Der p 1 purified from Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus
14.4
-
 (2-aminobenzoyl)-Val-Ala-norLeu-Ser-Tyr(3-NO2)-Asp recombinant Der p 1, expressed in Pichia pastoris Dermatophagoides pteronyssinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 recombinant Der p 1 and Der p 1 purified from Dermatophagoides pteronyssinus show differences in glycosylation Dermatophagoides pteronyssinus
25000
-
 gel filtration, Der p 1 purified from Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus
36000 60000 smear from 36 to 60 kDa, SDS-PAGE, recombinant Der p 1, expressed in Pichia pastoris Dermatophagoides pteronyssinus
55000
-
 average molecular weight, gel filtration, recombinant Der p 1, expressed in Pichia pastoris Dermatophagoides pteronyssinus

Organism

Organism UniProt Comment Textmining
Dermatophagoides pteronyssinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dermatophagoides pteronyssinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-aminobenzoyl)-Val-Ala-norLeu-Ser-Tyr(3-NO2)-Asp + H2O ADZ 50,059 Dermatophagoides pteronyssinus ?
-
 ?
N-Bz-Phe-Val-Arg-p-nitroanilide + H2O
-
 Dermatophagoides pteronyssinus ?
-
 ?

Synonyms

Synonyms Comment Organism
Der p 1 automaturation product of proDer p 1 (proDer p 1.0105 isoform) Dermatophagoides pteronyssinus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000051
-
 recombinant Der p 1, expressed in Pichia pastoris Dermatophagoides pteronyssinus Der p 1 prodomain
0.0000569
-
 Der p 1 purified from Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus Der p 1 prodomain