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Literature summary for 3.4.22.65 extracted from

  • Takai, T.; Kato, T.; Sakata, Y.; Yasueda, H.; Izuhara, K.; Okumura, K.; Ogawa, H.
    Recombinant Der p 1 and Der f 1 exhibit cysteine protease activity but no serine protease activity (2005), Biochem. Biophys. Res. Commun., 328, 944-952.
    View publication on PubMed
Search for more literature for 3.4.22.65

Application

Application Comment Organism
medicine involved in the pathogenesis of allergy Dermatophagoides pteronyssinus

Cloned(Commentary)

Cloned (Comment) Organism
preparation described elsewhere, proform secreted in Pichia pastoris culture supernatant and converted into their prosequence-removed mature form, recombinant protein exhibits cysteine protease activity but no serine protease activity Dermatophagoides pteronyssinus

Protein Variants

Protein Variants Comment Organism
N52Q mutant without glycosylation Dermatophagoides pteronyssinus

Inhibitors

Inhibitors Comment Organism Structure
E-64 irreversible inhibition, cysteine protease-specific Dermatophagoides pteronyssinus
additional information not inhibited by AEBSF Dermatophagoides pteronyssinus

Organism

Organism UniProt Comment Textmining
Dermatophagoides pteronyssinus
-
 house dust mite
-

Purification (Commentary)

Purification (Comment) Organism
proform secreted in Pichia pastoris culture supernatant and converted into their prosequence-removed mature form, anion exchange column and gel filtration Dermatophagoides pteronyssinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyloxycarbonyl-Gln-Ala-Arg-MCA + H2O
-
 Dermatophagoides pteronyssinus butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
butyloxycarbonyl-Gln-Gly-Arg-MCA + H2O
-
 Dermatophagoides pteronyssinus butyloxycarbonyl-Gln-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
butyloxycarbonyl-Phe-Ser-Arg-MCA + H2O
-
 Dermatophagoides pteronyssinus butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
butyloxycarbonyl-Val-Leu-Lys-MCA + H2O
-
 Dermatophagoides pteronyssinus butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
 ?
succinyl-Leu-Leu-Val-Tyr-MCA + H2O
-
 Dermatophagoides pteronyssinus succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
Der p1
-
 Dermatophagoides pteronyssinus
mite major group 1 allergens
-
 Dermatophagoides pteronyssinus

Cofactor

Cofactor Comment Organism Structure
additional information dithiothreitol dependent proteolytic activity Dermatophagoides pteronyssinus