Cloned (Comment) | Organism |
---|---|
wild-type pro-Der f1 is secreted to culture medium of baculovirus-transfeceted SF9 cells, pro-N53Q is not secreted in insect Sf9 cells although secreted in Pichia pastoris | Dermatophagoides farinae |
Protein Variants | Comment | Organism |
---|---|---|
C104S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C118S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C32S/C72S/N63Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C32S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C35S/N53Q | proform of mutant enzyme is efficiently secreted into culture medium. The secreted pro-C35S/N53Q is converted to the mature form by treatment in acidic conditions. Cysteine protease activity of the processed C35S/N53Q is lower than that of native Der f1 but significantly higher than that of the proenzyme | Dermatophagoides farinae |
C4S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C66S/C104S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C66S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
C72S/N53Q | proform of mutant enzyme is not efficiently secreted into culture medium | Dermatophagoides farinae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dermatophagoides farinae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation is essential for secretion in insect Sf9 cells but not in Pichia pastoris | Dermatophagoides farinae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | wild-type pro-Der f1 is secreted to culture medium of baculovirus-transfeceted SF9 cells, but pro-N53Q is not | Dermatophagoides farinae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | - |
Dermatophagoides farinae | butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? |