Literature summary for 3.4.22.65 extracted from

Yasuhara, T.; Takai, T.; Yuuki, T.; Okudaira, H.; Okumura, Y.
Biologically active recombinant forms of a major house dust mite group 1 allergen Der f 1 with full activities of both cysteine protease and IgE binding (2001), Clin. Exp. Allergy, 31, 116-124.

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Application

Application	Comment	Organism
medicine	the efficient system to prepare active recombinant enzyme is useful for diagnosis and standardized allergen-specific immunotherapy for house dust mite allergy. The system would by a tool for analysis of IgE epitopes, determination of tertiary structure, allergen engineering for safer and more effective allergen-specific immunotherapy	Dermatophagoides farinae

Cloned(Commentary)

Cloned (Comment)	Organism
the preproforms of Der f 1 and a mutant N53Q, whose consensus motif for N-glycosylation is disrupted, are expressed in Pichia pastoris	Dermatophagoides farinae

Protein Variants

Protein Variants	Comment	Organism
N53Q	the proform of N53Q is efficiently secreted into culture supernatant and processed to be of almost the same molecular weight as native enzyme by treatment in acidic conditions	Dermatophagoides farinae

Organism

Organism	UniProt	Comment	Textmining
Dermatophagoides farinae	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the wild-type recombinant enzyme is more highly glycosylated than the native enzyme	Dermatophagoides farinae
proteolytic modification	the N-terminal sequences of the secreted proteins are determined to start from -59 or -78, downstream from the putative signal-cleavage site in the prosequence of Der f 1. Those of processed proteins are determined to start from -2 or 1, two residues upstream from or at the N-terminus of native Der f 1. The prosequence is removed autocatalytically by dialysis against acidic buffer	Dermatophagoides farinae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O	-	Dermatophagoides farinae	butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin	-	?
butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O	-	Dermatophagoides farinae	butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin	-	?

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Release 2023.1 (January 2023)