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Literature summary for 3.4.22.65 extracted from

  • Pernas, M.; Sanchez-Ramos, I.; Sanchez-Monge, R.; Lombardero, M.; Arteaga, C.; Castanera, P.; Salcedo, G.
    Der p 1 and Der f 1, the highly related and major allergens from house dust mites, are differentially affected by a plant cystatin (2000), Clin. Exp. Allergy, 30, 972-978.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
chestnut cystatin inhibits the enzyme and greatly increases the larval mortality Dermatophagoides farinae
E-64
-
Dermatophagoides farinae
E-64
-
Dermatophagoides pteronyssinus
egg-white cystatin
-
Dermatophagoides farinae
additional information Der f1 from Dermatophagoides farinae is highly susceptible, Der p 1 from Dermatophagoides pteronyssinus is not affected by chestnut cystatin. Der p 1 inactivates chestnut chystatin by a specific proteolytic cleavage between Gly6 and Val7 Dermatophagoides pteronyssinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
casein pH 6.5, 37°C Dermatophagoides pteronyssinus
0.033
-
casein pH 6.5, 30°C Dermatophagoides farinae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Dermatophagoides farinae allergen from Dermatophagoides farinae ?
-
?

Organism

Organism UniProt Comment Textmining
Dermatophagoides farinae
-
-
-
Dermatophagoides pteronyssinus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Dermatophagoides pteronyssinus ?
-
?
casein + H2O
-
Dermatophagoides farinae ?
-
?
casein + H2O
-
Dermatophagoides pteronyssinus ?
-
?
chestnut cystatin + H2O specific proteolytic cleavage between Gly6 and Val7, giving rise to a noninhibitory processed protein. Not cleaved by Der f 1 from Dermatophygoides farinae Dermatophagoides pteronyssinus ?
-
?
additional information allergen from Dermatophagoides farinae Dermatophagoides farinae ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000031
-
chestnut cystatin pH 6.5, 30°C Dermatophagoides farinae