Application | Comment | Organism |
---|---|---|
pharmacology | studies on mechanisms of apoptosis induced by ionizing radiation in human leukaemic cells with a different status of p53 (TP53 tumor suppressor gene) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-3 + H2O | Homo sapiens | mechanisms of apoptosis induced by ionizing radiation on cell lines with a different status of p53 (TP53 tumor suppressor gene), extrinsic (caspase-8 dependent) and intrinsic (caspase-9 dependent) pathways activated | caspase-3 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P55211 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HL-60 cell | null mutant of protein p53 | Homo sapiens | - |
MOLT-4 cell | intact protein p53 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mechanisms of apoptosis induced by ionizing radiation on cell lines with a different status of p53 (TP53 tumor suppressor gene), activity of initial caspases (-8 and -9) analyzed by Western-Blot of whole-cell lysates and mitochondrial fractions, cells lacking the p53 protein show activation of caspase-8 prior to activation of caspase-9, cells harboring the p53 protein show a simultaneous activation of both initial caspases, extrinsic (caspase-8 dependent) and intrinsic (caspase-9 dependent) pathways activated upon exposure ionizing radiation regardless to the status of p53 protein | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-3 + H2O | mechanisms of apoptosis induced by ionizing radiation on cell lines with a different status of p53 (TP53 tumor suppressor gene), extrinsic (caspase-8 dependent) and intrinsic (caspase-9 dependent) pathways activated | Homo sapiens | caspase-3 + ? | - |
? |