Literature summary for 3.4.22.62 extracted from

Lee, H.J.; Lee, H.J.; Lee, E.O.; Ko, S.G.; Bae, H.S.; Kim, C.H.; Ahn, K.S.; Lu, J.; Kim, S.H.
Mitochondria-cytochrome C-caspase-9 cascade mediates isorhamnetin-induced apoptosis (2008), Cancer Lett., 270, 342-353.

Search for more literature for 3.4.22.62

Application

Application	Comment	Organism
pharmacology	in vitro analysis of apoptotic mechanism of isorhamnetin in Lewis lung cancer (LLC) cells, in vivo anti-cancer efficacy	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
benzyloxycarbonyl-LEHD-fluoromethylketone	i.e. z-LEHD-FMK, specific caspase-9 inhibitor, concentration of 50 microM, inhibitor effect on isorhamnetin-induced apoptosis, cells exposed to DMSO or 40 microM isorhamnetin for 48 h	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mus musculus	mitochondria-dependent caspase activation for mediation of isorhamnetin-induced apoptosis	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q9R0T0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Lewis lung carcinoma cell	isolated from 5-week-old female C57BL/6 mice, intraperitoneal injection of isorhamnetin (0.1 or 0.5 mg/kg) four days after tumor inoculation	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	apoptotic mechanism of the flavanoid isorhamnetin, isorhamnetin induces mitochondria-dependent caspase activation cascade, caspase inhibitors block isorhamnetin-induced apoptosis, increased cleavage of caspase-9 shown by Western blot analyses of isorhamnetin-treated cells, cytotoxicity assay, TUNEL assay, DNA fragmentation assay, measurement of mitochondrial membrane potential	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	mitochondria-dependent caspase activation for mediation of isorhamnetin-induced apoptosis	Mus musculus	?	-	?

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Release 2023.1 (January 2023)