Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of a leucine-zipper-linked dimeric caspase-9 (LZ-C9). Removal of the caspase recruitment domain of procaspase-9 and replacement with the leucine-zipper dimerization domain of the transcriptional factor GCN4. A six residue linker is added in between the leucine zipper and the beginning of the catalytic domain of caspase-9. LZ-C9 is more active than the caspase-9 holoenzyme for acetyl-LEHD-7-amido-4-trifluoromethylcoumarin but much less active the the caspase-9 holoenzyme for the physiological substrate procaspase-3 | Homo sapiens |
additional information | engineering of a leucine-zipper-linked dimeric caspase-9 (LZ-C9). Removal of the caspase recruitment domain of procaspase-9 and replacement with the leucine-zipper dimerization domain of the transcriptional factor GCN4. A six residue linker is added in between the leucine zipper and the beginning of the catalytic domain of caspase-9. LZ-C9 is more active than the caspase-9 holoenzyme for acetyl-LEHD-7-amido-4-trifluoromethyl coumarin but much less active the the caspase-9 holoenzyme for the physiological substrate procaspase-3 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.139 | - |
procaspase-3 | caspase-9 holoenzyme | Homo sapiens | |
0.466 | - |
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin | leucine-zipper-linked dimeric caspase-9 | Homo sapiens | |
0.466 | - |
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin | leucine-zipper-linked dimeric caspase-9 | Homo sapiens | |
0.686 | - |
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin | caspase-9 holoenzyme | Homo sapiens | |
0.686 | - |
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin | caspase-9 holoenzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procaspase-3 + H2O | Homo sapiens | - |
caspase-3 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P55211 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin + H2O | - |
Homo sapiens | acetyl-LEHD + 7-amino-4-trifluoromethyl coumarin | - |
? | |
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | acetyl-LEHD + 7-amino-4-trifluoromethylcoumarin | - |
? | |
procaspase-3 + H2O | - |
Homo sapiens | caspase-3 + ? | - |
? |