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Literature summary for 3.4.22.61 extracted from

  • Boatright, K.M.; Deis, C.; Denault, J.B.; Sutherlin, D.P.; Salvesen, G.S.
    Activiation of caspases-8 and -10 by FLIP L (2004), Biochem. J., 382, 651-657.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.61

Activating Compound

Activating Compound Comment Organism Structure
Fas-associated death domain protein-like interleukin-1-beta-converting enzyme-like inhibitory protein, long form FLIP L, results in a heterodimeric enzyme Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0206
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin cleaved caspase-8, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate Homo sapiens
0.0293
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin non-cleavable caspase-8 + 0.001 mM FLIP D297A, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate Homo sapiens
0.0313
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin non-cleavable caspase-8 + 0.001 mM FLIP, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate Homo sapiens
0.0552
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin cleaved caspase-8, 37°C, pH 7.4 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 monomer, non-cleavable caspase-8, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q14790
-
-

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-IETD-7-amido-4-fluoromethylcoumarin + H2O
-
 Homo sapiens acetyl-IETD + 7-amino-4-fluoromethylcoumarin
-
 ?
acetyl-Ile-Glu-Thr-Asp-7-amino-4-fluoromethylcoumarin + H2O 37°C, pH 7.4 Homo sapiens Ac-IETD + 7-amino-4-fluoromethylcoumarin
-
 ?

Subunits

Subunits Comment Organism
dimer active form, SDS-PAGE, gel filtration Homo sapiens
monomer inactive form, SDS-PAGE, gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
caspase-8
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.65
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin cleaved caspase-8, 37°C, pH 7.4 Homo sapiens
0.78
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin non-cleavable caspase-8 + 0.001 mM FLIP D297A, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate Homo sapiens
0.98
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin non-cleavable caspase-8 + 0.001 mM FLIP, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate Homo sapiens
1.25
-
 acetyl-IETD-7-amido-4-fluoromethylcoumarin cleaved caspase-8, 37°C, pH 7.4, in the presence of 0.7 M sodium citrate Homo sapiens