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Literature summary for 3.4.22.60 extracted from

  • Boucher, D.; Blais, V.; Denault, J.B.
    Caspase-7 uses an exosite to promote poly(ADP ribose) polymerase 1 proteolysis (2012), Proc. Natl. Acad. Sci. USA, 109, 5669-5674.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D23A the mutation shows increased catalytic activity but is unable to cleave PARP Homo sapiens
K38A the mutant shows increased catalytic activity Homo sapiens
K40A the mutant shows reduced catalytic activity Homo sapiens
K41A the mutant shows reduced catalytic activity Homo sapiens
S37A the mutant shows increased catalytic activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Z-VAD-fluoromethylketone irreversible inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0298
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
0.0319
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
0.0357
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
0.0366
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
0.0375
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
0.0478
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
0.0687
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme D23A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme does not cleave ICAD ?
-
 ?
p23 + H2O Homo sapiens better substrate for caspase-7 than caspase-3. p23 is cleaved at site PEVD*G ?
-
 ?
poly(ADP ribose) polymerase 1 + H2O Homo sapiens better substrate for caspase-7 than caspase-3, PARP-1 is cleaved at the canonical caspase-3/7 site DEVD*G ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55210
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not cleave ICAD Homo sapiens ?
-
 ?
additional information no activity with N-acetyl-PEVD-7-amido-4-fluoromethylcoumarin Homo sapiens ?
-
 ?
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O
-
 Homo sapiens N-acetyl-DEVD + 7-amino-4-fluoromethylcoumarin
-
 ?
p23 + H2O better substrate for caspase-7 than caspase-3. p23 is cleaved at site PEVD*G Homo sapiens ?
-
 ?
poly(ADP ribose) polymerase 1 + H2O better substrate for caspase-7 than caspase-3, PARP-1 is cleaved at the canonical caspase-3/7 site DEVD*G Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Casp7
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
3.4
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
3.9
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
4.1
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
4.3
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
4.4
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
9.5
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme D23A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
86
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
97
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
110
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
110
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
120
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
120
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens
140
-
 N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin mutant enzyme D23A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C Homo sapiens