Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D198A | additionally, amino acid residues 20-25 are replaced with a thrombin cleavage site (LVPRGS) and a thrombin cleavage site (LVPRGS) is inserted between Asp-203 and Thr-204 | Homo sapiens |
additional information | caspase cleavage site is substituted and a thrombin cleavage site inserted: amino acid residues 20-25 are replaced with a thrombin cleavage site (LVPRGS) and a thrombin cleavage site (LVPRGS) is inserted between Asp-203 and Thr-204 | Homo sapiens |
additional information | caspase cleavage sites are substituted: amino acid residues 20-25 and 195-200 are replaced with thrombin cleavage sites (LVPRGS) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0848 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin | Homo sapiens | |
0.111 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | wild-type protein | Homo sapiens | |
0.1193 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 | Homo sapiens | |
0.415 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin | Homo sapiens | |
0.9365 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Co2+) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O | - |
Homo sapiens | N-acetyl-Asp-Glu-Val-Asp + p-nitroaniline | - |
? | |
poly(ADP-ribose)polymerase + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
caspase-7 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 | Homo sapiens | |
0.1 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 | Homo sapiens | |
1.9 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin | Homo sapiens | |
3.6 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | wid-type protein | Homo sapiens | |
4.4 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 | Homo sapiens | |
0.83 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 | Homo sapiens | |
4.7 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin | Homo sapiens | |
33.3 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | wild-type protein | Homo sapiens | |
51.7 | - |
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide | mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin | Homo sapiens |