KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0605 | - |
acetyl-DEVD-7-amido-4-fluoromethylcoumarin | pH 7.2, 37°C | Homo sapiens | |
0.0646 | - |
acetyl-DEVD-4-nitroanilide | pH 7.2, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | human caspase-7 is not a nuclear caspase removal of the N-peptide does not allow an active transport or accumulation of human caspase-7 in the nuclei | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases | ? | - |
? | |
additional information | Homo sapiens | the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis | ? | - |
? | |
poly(ADP-ribose) polymerase + H2O | Homo sapiens | whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
GenBank Acc. No NM 001227 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the N-peptide of caspase-7 must be removed, probably be caspase-3, before efficient activation of the zymogen can occur in vivo. The N-peptide serves to physically sequester the caspase-7 zymogen in a cytosolic location that prevents access by upstream activators, caspase-8, caspase-9 and caspase-10 | Homo sapiens |
proteolytic modification | the 12000 Da and the 11000 Da polypeptides are generated by processing of the CMH-1 protein at Asp198-Ser199 and to a lesser extent at Asp206-Ala207 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-DEVD-4-nitroanilide + H2O | - |
Homo sapiens | acetyl-DEVD + 4-nitroaniline | - |
? | |
acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O | - |
Homo sapiens | acetyl-DEVD + 7-amino-4-fluoromethylcoumarin | - |
? | |
additional information | caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases | Homo sapiens | ? | - |
? | |
additional information | the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis | Homo sapiens | ? | - |
? | |
poly(ADP-ribose) polymerase + H2O | whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nucear entry | Homo sapiens | ? | - |
? | |
poly(ADP-ribose) polymerase + H2O | whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.9 | - |
acetyl-DEVD-7-amido-4-fluoromethylcoumarin | pH 7.2, 37°C | Homo sapiens | |
10.3 | - |
acetyl-DEVD-p-nitroanilide | pH 7.2, 37°C | Homo sapiens |