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Literature summary for 3.4.22.6 extracted from
- Chymopapain. I. Isolation, crystallization, and preliminary characterization (1962), J. Biol. Chem., 237, 1086-1094.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dimercaptopropanol | accelerates casein digestion | Carica papaya |  |
| Cys | accelerates casein digestion | Carica papaya | |
| glutathione | accelerates casein digestion | Carica papaya | |
| NaN3 | accelerates casein digestion | Carica papaya | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Carica papaya |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Carica papaya |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Carica papaya |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, pH 2.0, stable | Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Gly amide + H2O | weak activity | Carica papaya | benzoyl-Gly + NH3 | - |
? | |
| carbobenzoxy-L-Glu-L-Tyr + H2O | weak activity | Carica papaya | Carbobenzoxy-Glu + Tyr | - |
? | |
| casein + H2O | - |
Carica papaya | ? | - |
? | |
| L-Leu amide + H2O | weak activity | Carica papaya | Leu + NH3 | - |
? | |
| L-Leu-L-Tyr + H2O | weak activity | Carica papaya | Leu + Tyr | - |
? | |
| N-acetyl-L-Phe-L-Tyr + H2O | weak activity | Carica papaya | ? | - |
? | |
| N-benzoyl-L-Arg ethyl ester + H2O | - |
Carica papaya | N-benzoyl-Arg + ethanol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
half-life at pH 2.1: 2 min, half-life at pH 7.2: 75 min | Carica papaya |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
casein or hemoglobin digestion | Carica papaya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.8 | about 65% of maximal activity at pH 6.0 and at pH 9.8 | Carica papaya |
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