Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.6 extracted from

  • Cornell, H.J.; Doherty, W.; Stelmasiak, T.
    Papaya latex enzymes capable of detoxification of gliadin (2009), Amino Acids, 38, 155-165.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
fraction is estimated from plots of Kav against molecular weight using dextran blue, human gamma-globulin, bovine serum albumin and trypsin inhibitor as standards, mass spectrometric analysis of trypsin-digested fractions from chromatography are carried out by liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry in positive mode Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-
Carica papaya P14080
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Carica papaya
enzyme is semi-purified with from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Carica papaya
-
latex estimated as a fraction of papaya latex Carica papaya
-

Synonyms

Synonyms Comment Organism
Chymopapain
-
Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
enzyme is dissolved in phosphate buffer and used with a suspension of lysosomes in 0.154 mol/l sodium chloride Carica papaya