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Literature summary for 3.4.22.59 extracted from

  • Mintzer, R.; Ramaswamy, S.; Shah, K.; Hannoush, R.N.; Pozniak, C.D.; Cohen, F.; Zhao, X.; Plise, E.; Lewcock, J.W.; Heise, C.E.
    A whole cell assay to measure caspase-6 activity by detecting cleavage of lamin a/c (2012), PLoS ONE, 7, e30376.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.59

Application

Application Comment Organism
drug development the enzyme is a target for therapeutic intervention in Alzheimer's and Huntington's diseases Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of knockout mice by using a construct for targeting the C57BL/6 caspase-6 locus in ES cells. lamin A/C degradation is markedly reduced in fibroblasts extracted from caspase-6 knock-out mice relative to fibroblasts from wild type mice Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Ac-DEVD-CHO
-
 Homo sapiens
Ac-IETD-CHO
-
 Homo sapiens
Ac-VEID-CHO
-
 Homo sapiens
Q-VD-OPH a Pan caspase inhibitor Homo sapiens
Z-DEVD-fluoromethylketone
-
 Homo sapiens
Z-VAD-fluoromethylketone
-
 Homo sapiens
Z-VEID-fluoromethylketone
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lamin A + H2O Mus musculus
-
 ?
-
 ?
lamin A + H2O Homo sapiens
-
 ?
-
 ?
lamin A + H2O Mus musculus C57BL/6
-
 ?
-
 ?
lamin c + H2O Mus musculus
-
 ?
-
 ?
lamin c + H2O Homo sapiens
-
 ?
-
 ?
lamin c + H2O Mus musculus C57BL/6
-
 ?
-
 ?
additional information Mus musculus development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview ?
-
 ?
additional information Homo sapiens development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview ?
-
 ?
additional information Mus musculus C57BL/6 development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Mus musculus C57BL/6
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation active recombinant caspase-6 Homo sapiens
-
neuroblastoma cell
-
 Homo sapiens
-
SK-N-AS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ac-VEID)2-Rh110 + H2O
-
 Homo sapiens ?
-
 ?
lamin A + H2O
-
 Mus musculus ?
-
 ?
lamin A + H2O
-
 Homo sapiens ?
-
 ?
lamin A + H2O
-
 Mus musculus C57BL/6 ?
-
 ?
lamin c + H2O
-
 Mus musculus ?
-
 ?
lamin c + H2O
-
 Homo sapiens ?
-
 ?
lamin c + H2O
-
 Mus musculus C57BL/6 ?
-
 ?
additional information development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview Mus musculus ?
-
 ?
additional information development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview Homo sapiens ?
-
 ?
additional information development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview Mus musculus C57BL/6 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000162
-
 pH 7.2, 37°C, recombinant enzyme Homo sapiens Ac-VEID-CHO
0.0000305
-
 pH 7.2, 37°C, recombinant enzyme Homo sapiens Ac-DEVD-CHO
0.0000532
-
 pH 7.2, 37°C, recombinant enzyme Homo sapiens Ac-IETD-CHO
0.0001286
-
 pH 7.2, 37°C, recombinant enzyme Homo sapiens Z-VEID-fluoromethylketone
0.0004023
-
 pH 7.2, 37°C, recombinant enzyme Homo sapiens Z-DEVD-fluoromethylketone
0.002112
-
 pH 7.2, 37°C, recombinant enzyme Homo sapiens Z-VAD-fluoromethylketone

Expression

Organism Comment Expression
Homo sapiens staurosporine induces caspase-6 up

General Information

General Information Comment Organism
physiological function caspase-6 is a cysteinyl protease implicated in neurodegenerative conditions including Alzheimer's and Huntington's diseases Homo sapiens