Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.59 extracted from

  • Lee, A.W.; Champagne, N.; Wang, X.; Su, X.D.; Goodyer, C.; Leblanc, A.C.
    Alternatively spliced caspase-6B isoform inhibits the activation of caspase-6A (2010), J. Biol. Chem., 285, 31974-31984.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.59

Activating Compound

Activating Compound Comment Organism Structure
RCasp1 mediates increased self-processing of pro-Casp6a into its active subunits Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
the CASP6 gene generates an alternatively spliced transcript known as CASP6beta in addition to the CASP6alpha transcript that encodes pro-Casp6a. Individual expression of His-tagged pro-Casp6b and of catalytic mutant pro-Casp6aC163A in Escherichia coli strain BL21(DE3), Expression of pro-Casp6b from the pCep4beta construct in SK-N-SH cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C163A catalytically inactive mutant pro-Casp6aC163A Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information alternatively spliced caspase-6B isoform inhibits the proteolytic self-activation of caspase-6A in vitro and in cells. But purified bacterially expressed recombinant pro-Casp6b does not possess catalytic activity and does not inhibit the active form of Casp6a Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 34000, pro-Casp6a, SDS-PAGE, x * 23000, pro-Casp6b, SDS-PAGE Homo sapiens
34000
-
 x * 34000, pro-Casp6a, SDS-PAGE, x * 23000, pro-Casp6b, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification pro-forms of caspase-6 isozymes need to be proteolytically self-activated. RCasp1-mediated increased processing of pro-Casp6a, while pro-Casp6b inhibits pro-Casp6a activationinto its active subunits Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged pro-Casp6b from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant catalytic mutant pro-Casp6aC163A from Escherichia coli strain BL21(DE3) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
BE(2)-M17 cell
-
 Homo sapiens
-
brain temporal cortex, frontal cortex, and cerebellar tissues Homo sapiens
-
HCT-116 cell
-
 Homo sapiens
-
HEK-293T cell
-
 Homo sapiens
-
MC-IXC cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
neuron
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-VEID-4-trifluoromethylcoumarin 7-amide + H2O
-
 Homo sapiens Ac-VEID + 7-amino-4-trifluoromethylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, pro-Casp6a, SDS-PAGE, x * 23000, pro-Casp6b, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
caspase-6A
-
 Homo sapiens
caspase-6B
-
 Homo sapiens
VEIDase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information the pro-Casp6b protein resulting from CASP6beta translation lacks part of the pro-domain and part of the large p20 subunit of pro-Casp6a Homo sapiens
physiological function alternatively spliced caspase-6B isoform inhibits the activation of caspase-6A. Pro-Casp6b can negatively regulate pro-Casp6a activation in neurons and prevent Casp6a-mediated axonal degeneration. Pro-Casp6b inhibits RCasp1-mediated processing of Pro-Casp6a in vitro Homo sapiens