Cloned (Comment) | Organism |
---|---|
cloning of full-length cDNA of Lyccasp3, DNA and amino acid sequence determination and analysis, phylogenetic tree, real-time PCR expression analysis, functional recombinant expression in Escherichia coli strain BL21 (DE3) | Larimichthys crocea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Larimichthys crocea | C6ZH48 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Larimichthys crocea | - |
gill | - |
Larimichthys crocea | - |
heart | - |
Larimichthys crocea | - |
intestine | - |
Larimichthys crocea | - |
kidney | - |
Larimichthys crocea | - |
liver | - |
Larimichthys crocea | - |
additional information | ubiquitous, constitutive expression with varying expression levels from tissue to tissue, real-time PCR expression analysis | Larimichthys crocea | - |
muscle | - |
Larimichthys crocea | - |
spleen | - |
Larimichthys crocea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-DEVD-4-nitroanilide + H2O | an artificial caspase-3 substrate | Larimichthys crocea | Ac-DEVD + 4-nitroaniline | - |
? | |
Ac-IETD-4-nitroanilide + H2O | an artificial caspase-8 substrate, low activity | Larimichthys crocea | Ac-IETD + 4-nitroaniline | - |
? | |
Ac-LEHD-4-nitroanilide + H2O | an artificial caspase-9 substrate, low activity | Larimichthys crocea | Ac-LEHD + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Lyccasp3 exhibits a conserved caspase-3 architecture including a prodomain, a large subunit and a small subunit | Larimichthys crocea |
Synonyms | Comment | Organism |
---|---|---|
Lyccasp3 | - |
Larimichthys crocea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Larimichthys crocea |
Organism | Comment | Expression |
---|---|---|
Larimichthys crocea | quick induction fo expression in spleen and kidney by stimulation with either poly(I:C) or inactivated trivalent bacterial vaccine | up |
General Information | Comment | Organism |
---|---|---|
evolution | caspases-3 is a member of the cysteine-aspartic acid protease (caspase) family, fish caspase-3 subgroup. Lyccasp3 exhibits a conserved caspase-3 architecture including a prodomain, a large subunit and a small subunit. Several residues critical in the caspase-3 catalytic centre and binding pocket, as well as the active-site pentapeptide motif Q172ACRG176, are present in the deduced Lyccasp3 sequence, phylogenetic tree, overview | Larimichthys crocea |
metabolism | the intrinsic apoptotic pathway, which is initiated by caspase-9 and executed by caspase-3, is activated during the immune response induced by poly(I:C) or bacterial vaccine in the fish | Larimichthys crocea |