Cloned (Comment) | Organism |
---|---|
GFP-tagged subunit and enzyme expression, the small subunit as well as the full-length caspase-3 display evident cytoplasm localization, while the large subunit and its truncated forms are distributed throughout the whole cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R207E/C163S | site-directed mutagenesis, the mutant resides in the cytoplasm | Homo sapiens |
R64E | site-directed mutagenesis, the mutant existed in the nuclear fraction, the subcellular localization signal in the rev-caspase-3 is not disrupted by the R64E mutation | Homo sapiens |
R64E/C163S | site-directed mutagenesis, the mutant resides in the cytoplasm | Homo sapiens |
R64E/R207E | site-directed mutagenesis, the mutant resides in the cytoplasm | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | pro-enzyme | Homo sapiens | 5737 | - |
additional information | p3-mediated cleavage activity abrogates the functions of other crm-1-dependent nuclear export signals with low nuclear export signal activity | Homo sapiens | - |
- |
nucleus | activated enzyme, the enzyme harbors one crm-1-independent nuclear export signal but no nuclear localization signal residing at the small subunit of caspase-3. The nuclear entry of active caspase-3 is not mediated by passive diffusion, but by its p3-recognition-based specific cleavage activity. The p3-mediated cleavage activity abrogates the function of the nuclear localization signal in caspase-3, which is essential for the active caspase-3 to enter into the nucleus | Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
General Information | Comment | Organism |
---|---|---|
malfunction | mutations disrupting the cleavage activity or the p3 recognition site cause a defect in the nuclear entry of active caspase-3 | Homo sapiens |
physiological function | caspase-3 exists in the form of pro-enzyme, and is located predominantly in the cytoplasm of cells. During apoptosis, caspase-3 is cleaved and activated by upstream caspases, and is translocated into the nucleus to cleave its nuclear substrates, resulting in characteristic apoptotic nuclear changes such as DNA fragmentation, chromatin condensation and nuclear disruption | Homo sapiens |