Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FADD | adapter molecule, required for caspase-3 activation | Mus musculus | |
FADD | adapter molecule, required for caspase-3 activation | Homo sapiens | |
additional information | In some cells, active caspase-8 is sufficient to activate caspase-3 directly. In other cells, however, caspase-8 indirectly mediates caspase-3 activation by cleaving the proapoptotic Bcl-2 family member Bid, which induces mitochondrial cytochrome c release. Leptospira interrogans induces apoptosis in macrophages and hepatocytes via caspase-8- and caspase-3-dependent pathways | Mus musculus | |
additional information | In some cells, active caspase-8 is sufficient to activate caspase-3 directly. In other cells, however, caspase-8 indirectly mediates caspase-3 activation by cleaving the proapoptotic Bcl-2 family member Bid, which induces mitochondrial cytochrome c release. Leptospira interrogans induces apoptosis in macrophages and hepatocytes via caspase-8- and caspase-3-dependent pathways | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 activation | Homo sapiens | |
additional information | inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 activation | Mus musculus | |
N-carbobenzyloxy-DEVD-fluoromethyl ketone | effects on the activation of caspase-3 through cell infection with Leptospira interrogans | Homo sapiens | |
N-carbobenzyloxy-DEVD-fluoromethyl ketone | effects on the activation of caspase-3 through cell infection with Leptospira interrogans | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADDcaspase-8-dependent pathway, independently of mitochondrial cytochrome ccaspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview | ? | - |
? | |
additional information | Homo sapiens | caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADDcaspase-8-dependent pathway, independently of mitochondrial cytochrome ccaspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview | ? | - |
? | |
procaspase-6 + H2O | Mus musculus | activation requires caspase-3 | caspase-6 + ? | - |
? | |
procaspase-6 + H2O | Homo sapiens | activation requires caspase-3 | caspase-6 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | caspase-9 and caspase-8 activate procaspase-3 to caspase-3 by cleavage | Mus musculus |
proteolytic modification | caspase-9 and caspase-8 activate procaspase-3 to caspase-3 by cleavage | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
hepatocyte | - |
Mus musculus | - |
hepatocyte | - |
Homo sapiens | - |
J-774A.1 cell | - |
Mus musculus | - |
macrophage | peritoneal | Mus musculus | - |
macrophage | peritoneal | Homo sapiens | - |
umbilical vein endothelial cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin + H2O | - |
Mus musculus | acetyl-Asp-Glu-Val-Asp + 7-amino-4-trifluoromethylcoumarin | - |
? | |
acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | acetyl-Asp-Glu-Val-Asp + 7-amino-4-trifluoromethylcoumarin | - |
? | |
additional information | caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADDcaspase-8-dependent pathway, independently of mitochondrial cytochrome ccaspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview | Mus musculus | ? | - |
? | |
additional information | caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADDcaspase-8-dependent pathway, independently of mitochondrial cytochrome ccaspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview | Homo sapiens | ? | - |
? | |
procaspase-6 + H2O | activation | Mus musculus | caspase-6 + ? | - |
? | |
procaspase-6 + H2O | activation | Homo sapiens | caspase-6 + ? | - |
? | |
procaspase-6 + H2O | activation requires caspase-3 | Mus musculus | caspase-6 + ? | - |
? | |
procaspase-6 + H2O | activation requires caspase-3 | Homo sapiens | caspase-6 + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cysteine aspartic acid-specific protease | - |
Mus musculus |
cysteine aspartic acid-specific protease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |