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Literature summary for 3.4.22.56 extracted from

  • Fang, B.; Boross, P.I.; Tozser, J.; Weber, I.T.
    Structural and kinetic analysis of caspase-3 reveals role for S5 binding site in substrate recognition (2006), J. Mol. Biol., 360, 654-666.
    View publication on PubMed
Search for more literature for 3.4.22.56

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitors acetyl-Asp-Glu-Val-Asp-aldehyde, acetyl-Val-Asp-Val-Ala-Asp-aldehyde, acetyl-Asp-Met-Gln-Asp-aldehyde Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Acetyl-Asp-Glu-Val-Asp-aldehyde
-
 Homo sapiens
acetyl-Asp-Met-Gln-Asp-aldehyde
-
 Homo sapiens
acetyl-Val-Asp-Val-Ala-Asp-aldehyde
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
0.147
-
 acetyl-L-Leu-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
0.164
-
 acetyl-L-Val-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
0.222
-
 acetyl-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
1.6
-
 acetyl-L-Asp-L-Met-L-Gln-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P42574 recombinant enzyme
-

Reaction

Reaction Comment Organism Reaction ID
strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-/- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position hydrophobic S5 site, where the side-chains of F250 and F252 interact with substrate. Kinetic importance of P5 site Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-Asp-L-Glu-L-Val-L-Asp-4-nitroanilide + H2O
-
 Homo sapiens acetyl-L-Asp-L-Glu-L-Val-L-Asp + 4-nitroaniline
-
 ?
acetyl-L-Asp-L-Met-L-Gln-L-Asp-4-nitroanilide + H2O
-
 Homo sapiens acetyl-L-Asp-L-Met-L-Gln-L-Asp + 4-nitroaniline
-
 ?
acetyl-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide + H2O
-
 Homo sapiens acetyl-L-Asp-L-Val-L-Ala-L-Asp + 4-nitroaniline
-
 ?
acetyl-L-Leu-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide + H2O
-
 Homo sapiens acetyl-L-Leu-L-Asp-L-Val-L-Ala-L-Asp + 4-nitroaniline
-
 ?
acetyl-L-Val-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide + H2O
-
 Homo sapiens acetyl-L-Val-L-Asp-L-Val-L-Ala-L-Asp + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 acetyl-L-Val-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
0.84
-
 acetyl-L-Leu-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
0.88
-
 acetyl-L-Asp-L-Glu-L-Val-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
0.9
-
 acetyl-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens
3.5
-
 acetyl-L-Asp-L-Met-L-Gln-L-Asp-4-nitroanilide pH 7.5, 22°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000013
-
 Acetyl-Asp-Glu-Val-Asp-aldehyde pH 7.5, 22°C Homo sapiens
0.0000065
-
 acetyl-Val-Asp-Val-Ala-Asp-aldehyde pH 7.5, 22°C Homo sapiens
0.0000124
-
 acetyl-Asp-Met-Gln-Asp-aldehyde pH 7.5, 22°C Homo sapiens