Literature summary for 3.4.22.54 extracted from

Rey, M.A.; Davies, P.L.
The protease core of the muscle-specific calpain, p94, undergoes Ca2+-dependent intramolecular autolysis (2002), FEBS Lett., 532, 401-406.

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Cloned(Commentary)

Cloned (Comment)	Organism
the C-terminally truncated form of the enzyme that comprises the protease cor, domains I and II, alone with its insertion sequence, IS1, and N-terminal leader sequence, NS is expressed in Escherichia coli BL21(DE3)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	the C-terminally truncated form of the enzyme that comprises the protease core, domains I and II, alone with its insertion sequence, IS1, and N-terminal leader sequence, NS. This 47000 Da p94I-II minicalpain is stable during purification	Homo sapiens

General Stability

General Stability	Organism
isolation of the intact 94000 Da enzyme is difficult to achieve due to its rapid autolysis. The C-terminally truncated form of the enzyme that comprises the protease core, domains I and II, alone with its insertion sequence, IS1, and N-terminal leader sequence, NS. This 47000 Da p94I-II minicalpain is stable during purification. In the presence of Ca2+, p94I-II cleaves itself within the NS and IS1 sequences. Autolysis is an intramolecular event	Homo sapiens

Organism

isolation of the intact 94000 Da enzyme is difficult to achieve due to its rapid autolysis. The C-terminally truncated form of the enzyme that comprises the protease core, domains I and II, alone with its insertion sequence, IS1, and N-terminal leader sequence, NS. This 47000 Da p94I-II minicalpain is stable during purification. In the presence of Ca2+, p94I-II cleaves itself within the NS and IS1 sequences. Autolysis is an intramolecular event

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Homo sapiens	-

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Release 2023.1 (January 2023)