Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-NH2 | - |
Rattus norvegicus | |
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 | - |
Rattus norvegicus | |
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 | - |
Rattus norvegicus | |
Ac-Thr-Ser-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 | - |
Rattus norvegicus | |
Ac-Thr-Trp-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 | - |
Rattus norvegicus | |
additional information | not inhibited by Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-NH2, Ac-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Thr-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Ser-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Gln-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Val-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Thr-Pro-Pro-Pro-Ser-NH2, and Ac-Thr-Pro-Leu-alpha-azaglycine-Arg-Pro-Pro-Pro-Ser-NH2 | Rattus norvegicus | |
Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 3 mM used in assay conditions | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? | |
TPLKSPPPSPR + H2O | efficient substrate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
calpain 2 | - |
Rattus norvegicus |
m-calpain | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
Ac-Thr-Ser-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 | in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C | Rattus norvegicus | |
0.0058 | - |
Ac-Thr-Trp-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 | in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C | Rattus norvegicus | |
0.0072 | - |
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 | in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C | Rattus norvegicus | |
0.0087 | - |
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 | in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C | Rattus norvegicus | |
0.0203 | - |
Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 | in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C | Rattus norvegicus | |
0.0898 | - |
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-NH2 | in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C | Rattus norvegicus |