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Literature summary for 3.4.22.53 extracted from

  • Banoczi, Z.; Tantos, A.; Farkas, A.; Majer, Z.; Dokus, L.E.; Tompa, P.; Hudecz, F.
    New m-calpain substrate-based azapeptide inhibitors (2013), J. Pept. Sci., 19, 370-376.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-NH2
-
Rattus norvegicus
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2
-
Rattus norvegicus
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2
-
Rattus norvegicus
Ac-Thr-Ser-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2
-
Rattus norvegicus
Ac-Thr-Trp-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2
-
Rattus norvegicus
additional information not inhibited by Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-NH2, Ac-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Thr-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Ser-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Gln-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Val-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2, Ac-Thr-Pro-Leu-alpha-azaglycine-Thr-Pro-Pro-Pro-Ser-NH2, and Ac-Thr-Pro-Leu-alpha-azaglycine-Arg-Pro-Pro-Pro-Ser-NH2 Rattus norvegicus
Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 3 mM used in assay conditions Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
Rattus norvegicus N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
?
TPLKSPPPSPR + H2O efficient substrate Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
calpain 2
-
Rattus norvegicus
m-calpain
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0035
-
Ac-Thr-Ser-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
0.0058
-
Ac-Thr-Trp-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
0.0072
-
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
0.0087
-
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
0.0203
-
Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-Pro-Ser-Pro-Arg-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus
0.0898
-
Ac-Thr-Pro-Leu-alpha-azaglycine-Ser-Pro-Pro-NH2 in 10 mM HEPES, 150 mM NaCl, 1 mM EDTA, pH 7.5, at 22°C Rattus norvegicus