Literature summary for 3.4.22.52 extracted from

Diaz, B.G.; Gross, S.; Assfalg-Machleidt, I.; Pfeiler, D.; Gollmitzer, N.; Gabrijelcic-Geiger, D.; Stubbs, M.T.; Fritz, H.; Auerswald, E.A.; Machleidt, W.
Cystatins as calpain inhibitors: engineered chicken cystatin- and stefin B-kininogen domain 2 hybrids support a cystatin-like mode of interaction with the catalytic subunit of m-calpain (2001), Biol. Chem., 382, 97-107.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloning of the 21000 Da small subunit and expression in Escherichia coli BL384	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cystatin	engineered cystatins. Recombinant hybrids of human stefin B with KS2 and DELTAL110 deletion mutants of chicken cystatin-KD2 hybrids. Substitution of the N-terminal contact region of stefin B by ther corresponding KD2 sequence results in a calpain inhibitor with a Ki-value of 188 nM. Deletion of L110 improves inhibition 4 to 8fold. All engineered cystatins are temporary inhibitors	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of a 21000 Da calpain small subunit fragment	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	-	Homo sapiens	-

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Release 2023.1 (January 2023)