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Literature summary for 3.4.22.52 extracted from
- Molecular and catalytic characterization of intact heterodimeric and derived monomeric calpains isolated under different conditions from pig polymorphonuclear leukocytes (1988), Biochemistry, 27, 3260-3267.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dimeric calpain I requires 0.002 mM for half-maximal activation and 0.01 mM for maximal activation. The 70000 Da monomeric calpain I requires only 0.001 mM Ca2+ for half-maximal activity | Sus scrofa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE | Sus scrofa |
| 83000 | - |
1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| polymorphonuclear leukocyte | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Sus scrofa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the 70000 Da monomeric calpain I is much less heat stable than the parent heterodimeric calpain I | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
monomeric calpain I | Sus scrofa |
| 7.5 | - |
dimeric calpain I | Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | pH 6.5: about 35% of maximal activity, pH 8.0: about 50% of maximal activity | Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the 70000 Da monomeric calpain I is less pH stable than the parent heterodimeric calpain I | Sus scrofa |
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