Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K11777 | the chemotherapeutical drug targets the major cysteine protease cruzain and disrupts amastigote intracellular development. The mechanism of drug resistance, in primary epimastigotes, is due to secretion of inactive, unprocessed cruzain | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | the signaling factor NF-kB P65 colocalizes with cruzain on the cell surface of intracellular wild-type parasites | Trypanosoma cruzi | 9986 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
human NF-kappaB P65 + H2O | Trypanosoma cruzi | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human NF-kappaB P65 + H2O | - |
Trypanosoma cruzi | ? | - |
? |
General Information | Comment | Organism |
---|---|---|
malfunction | cruzain-deficient Trypanosoma cruzi rapidly activates host macrophages via NF-kB P65 and is unable to survive intracellularly within macrophages. No significant IL-12 expression occurs in macrophages infected with wild-type Trypanosoma cruzi and treated with lipopolysacchrides and brefeldin A, confirming impairment of macrophage activation pathways | Trypanosoma cruzi |
physiological function | infection with wild-type parasites appears to induce cruzain-mediated proteolysis of NF-kappaB P65 leading to unresponsiveness of the host macrophage during early 60 min of infection. This immune evasion mechanism may be critical for Trypaosoma cruzi survival during early natural infection with a low number of trypmastigotes. The signaling factor NF-kappaB P65 colocalizes with cruzain on the cell surface of intracellular wild-type parasites, and is proteolytically cleaved. Transcription factor NF-kappaB P65 is translocated to the nucleus and activated only in macrophages infected with cruzain-deficient parasites | Trypanosoma cruzi |