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Literature summary for 3.4.22.51 extracted from

  • Judice, W.A.; Puzer, L.; Cotrin, S.S.; Carmona, A.K.; Coombs, G.H.; Juliano, L.; Juliano, M.A.
    Carboxydipeptidase activities of recombinant cysteine peptidases. Cruzain of Trypanosoma cruzi and CPB of Leishmania mexicana (2004), Eur. J. Biochem., 271, 1046-1053.
    View publication on PubMed
Search for more literature for 3.4.22.51

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00095
-
 2-aminobenzoyl-RRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi
0.0013
-
 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 37°C, pH 5.5 Trypanosoma cruzi
0.0023
-
 2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Trp-OH 37°C, pH 5.5 Trypanosoma cruzi
0.0035
-
 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi
0.0036
-
 2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Pro-OH 37°C, pH 5.5 Trypanosoma cruzi
0.0039
-
 2-aminobenzoyl-FRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi
0.019
-
 2-aminobenzoyl-ARF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
 recombinantly expressed in Escherichia coli as a C-terminally truncated protein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-ARF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-FRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Pro-OH + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Trp-OH + H2O
-
 Trypanosoma cruzi ?
-
 ?
2-aminobenzoyl-RRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH + H2O
-
 Trypanosoma cruzi ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 2-aminobenzoyl-ARF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi
0.13
-
 2-aminobenzoyl-RRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi
1.4
-
 2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Pro-OH 37°C, pH 5.5 Trypanosoma cruzi
2.9
-
 2-aminobenzoyl-FRF-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi
3.4
-
 2-aminobenzoyl-Phe-Arg-(2,4-dinitrophenyl)-epsilon-NH2-lysine-Trp-OH 37°C, pH 5.5 Trypanosoma cruzi
4.2
-
 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 37°C, pH 5.5 Trypanosoma cruzi
5.5
-
 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 37°C, pH 5.5 Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH Trypanosoma cruzi
6 7 hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9 pH 3.5: about 65% of maximal activity, pH 6.5-9.0: about 55% of maximal activity, hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH Trypanosoma cruzi
4.5 9 pH 4.5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity, hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 Trypanosoma cruzi