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Literature summary for 3.4.22.51 extracted from
- Cruzipain, the major cysteine proteinase from the protozoan parasite Trypanosoma cruzi (1997), Biol. Chem., 378, 1-10.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Urea | activation at 2 M | Trypanosoma cruzi |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme lacking the C-terminal domain, complexed with benzyloxycarbonyl-Phe-Ala-fluoromethylketone | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzyloxycarbonyl-Phe-Ala-fluoromethylketone | - |
Trypanosoma cruzi | |
| chicken cystatin | - |
Trypanosoma cruzi | |
| chymostatin | - |
Trypanosoma cruzi | |
| Human cystatin C | - |
Trypanosoma cruzi | |
| L-kininogen | - |
Trypanosoma cruzi | |
| leupeptin | - |
Trypanosoma cruzi | |
| N-carbobenzoxy-FA-diazomethylketone | inhibition in vivo | Trypanosoma cruzi | |
| N-carbobenzoxy-FF-diazomethylketone | inhibition in vivo | Trypanosoma cruzi | |
| N-carbobenzoxy-FK-acyloxymethylketone | inhibition in vivo | Trypanosoma cruzi | |
| N-carbobenzoxy-VVR-4-nitroanilide | strong substrate inhibition | Trypanosoma cruzi | |
| Stefin A | - |
Trypanosoma cruzi | |
| Stefin B | - |
Trypanosoma cruzi | |
| [(S)-1-carboxy-2-phenylethyl]-carbamoyl-L-Arg-L-Val-arginal | - |
Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
N-carbobenzoxy-VVR-4-nitroanilide | pH 9 | Trypanosoma cruzi | |
| 0.066 | - |
benzoyl-PFR-4-nitroanilide | - |
Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Trypanosoma cruzi | 5764 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | Trypanosoma cruzi | - |
? | - |
? | |
| Hemoglobin + H2O | Trypanosoma cruzi | - |
? | - |
? | |
| Immunoglobulin G1 + H2O | Trypanosoma cruzi | - |
? | - |
? | |
| Immunoglobulin G3 + H2O | Trypanosoma cruzi | - |
? | - |
? | |
| additional information | Trypanosoma cruzi | antigenic character in natural human infections | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q598P5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme contains 10% carbohydrate | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| benzoyl-PFR-4-nitroanilide + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| Bovine serum albumin + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| casein + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| Hemoglobin + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| Immunoglobulin G1 + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| Immunoglobulin G1 + H2O | cleaves specifically at the hinge region | Trypanosoma cruzi | ? | - |
? | |
| Immunoglobulin G3 + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| Immunoglobulin G3 + H2O | cleaves specifically at the hinge region | Trypanosoma cruzi | ? | - |
? | |
| insulin A + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| insulin B + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| KEEASSAVVGGPG + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| KEEASSAVVRGPG + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| additional information | substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group | Trypanosoma cruzi | ? | - |
? | |
| additional information | antigenic character in natural human infections | Trypanosoma cruzi | ? | - |
? | |
| N-carbobenzoxy-Val-Val-Arg-4-nitroanilide + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| N-carbobenzoxy-VVR-4-nitroanilide + H2O | - |
Trypanosoma cruzi | N-carbobenzoxy-VVR + 4-nitroaniline | - |
? | |
| peptidyl-beta-naphthylamide + H2O | derivatives of | Trypanosoma cruzi | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
KEEASSAVVGGPG as substrate | Trypanosoma cruzi |
| 6.8 | - |
KEEASSAVVRGPG as substrate | Trypanosoma cruzi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable at alkaline pH | Trypanosoma cruzi |
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