Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.51 extracted from

  • Cazzulo, J.J.; Cazzulo Franke, M.C.; Martinez, J.; Franke de Cazzulo, B.M.
    Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi (1990), Biochim. Biophys. Acta, 1037, 186-191.
    View publication on PubMed
Search for more literature for 3.4.22.51

Activating Compound

Activating Compound Comment Organism Structure
guanidine hydrochloride activates at low concentrations but inhibits above 2 M Trypanosoma cruzi
KSCN activates at low concentrations but inhibits above 1 M Trypanosoma cruzi
Urea maximal at 2 M with natural substrates, no activation with synthetic substrates Trypanosoma cruzi

General Stability

General Stability Organism
unstable in the absence of substrate at pH 8.7 and 37°C Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride activates at low concentrations but inhibits above 2 M Trypanosoma cruzi
KSCN activates at low concentrations but inhibits above 1 M Trypanosoma cruzi
L-trans-epoxysuccinylleucylamido(4-guanidino)butane strong inhibitor Trypanosoma cruzi
Urea 70% inhibition at 5 M Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide
-
 Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000 60000 SDS-PAGE, depending on electrophoretic conditions Trypanosoma cruzi
36300
-
 calculated from nucleotide sequence Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q598P5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008 1.68 4-nitroanilide derivatives Trypanosoma cruzi
0.1 3.77 beta-naphthylamide derivatives Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Trypanosoma cruzi ?
-
 ?
benzoyl-PFR-4-nitroanilide + H2O
-
 Trypanosoma cruzi ?
-
 ?
additional information substrates are chromogenic peptides with a carboxyl Arg or Lys, enzyme requires at least one more amino acid, preferably Arg, Phe, Val or Leu between the terminal Arg or Lys and the amino-blocking group Trypanosoma cruzi ?
-
 ?
N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide + H2O
-
 Trypanosoma cruzi N-carbobenzoxy-Arg-Arg + 2-amino-4-methoxynaphthalene
-
 ?
peptidyl-4-nitroanilide + H2O derivatives of Trypanosoma cruzi ?
-
 ?
peptidyl-beta-naphthylamide + H2O derivatives of Trypanosoma cruzi ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 unstable in the absence of substrate at pH 8.7 Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0805
-
 N-carbobenzoxy-Arg-Arg-2-(4-methoxy)-naphthylamide
-
 Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay with azocasein Trypanosoma cruzi
7
-
 beta-naphthylamide derivatives as substrate Trypanosoma cruzi
9
-
 4-nitroanilide derivatives as substrate Trypanosoma cruzi

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.7
-
 unstable in the absence of substrate at 37°C Trypanosoma cruzi