Literature summary for 3.4.22.48 extracted from

Filipek, R.; Szczepanowski, R.; Sabat, A.; Potempa, J.; Bochtler, M.
Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition (2004), Biochemistry, 43, 14306-14315.

Search for more literature for 3.4.22.48

Cloned(Commentary)

Cloned (Comment)	Organism
prostaphopain B, expression as GST-fusion protein in Escherichia coli strain BL21(DE3)	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant mature staphopain B, sitting drop vapour diffusion method at 4°C or 21°C, equal volume of protein solution containing 15 mg/ml protein in 5mM Tris, pH 7.5, and reservoir solution containing 50 mM Bis-Tris, pH 6.5, 20% w/v PEG 4000, or 100 mM HEPES, pH 7.5, 21% w/v PEG 4000, and 10% v/v 2-propanol, are mixed, a few weeks, X-ray diffraction structure determination and analysis at 2.5-2.8 A resolution	Staphylococcus aureus

Crystallization (Comment)

Organism

purified recombinant mature staphopain B, sitting drop vapour diffusion method at 4°C or 21°C, equal volume of protein solution containing 15 mg/ml protein in 5mM Tris, pH 7.5, and reservoir solution containing 50 mM Bis-Tris, pH 6.5, 20% w/v PEG 4000, or 100 mM HEPES, pH 7.5, 21% w/v PEG 4000, and 10% v/v 2-propanol, are mixed, a few weeks, X-ray diffraction structure determination and analysis at 2.5-2.8 A resolution

Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the proregion of the zymogen is inhibitory for the mature enzyme	Staphylococcus aureus
staphostatin	-	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus V8	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	staphopain is synthesized as proenzyme, which is processed to the mature form by cleavage of the N-terminally propeptide, interactions between the propeptide and the mature enzyme, modeling, overview	Staphylococcus aureus

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-fusion prostaphopain B from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, gel filtration, and cleavage of the GST-tag followed by ultrafiltration	Staphylococcus aureus

Reaction

Reaction	Comment	Organism	Reaction ID
broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate	active site and substrate binding subsite structures	Staphylococcus aureus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Bz-Pro-Phe-Arg-4-nitroanilide + H2O	chromogenic substrate	Staphylococcus aureus	Bz-Pro-Phe-Arg + 4-nitroaniline	-	?
Bz-Pro-Phe-Arg-4-nitroanilide + H2O	chromogenic substrate	Staphylococcus aureus V8	Bz-Pro-Phe-Arg + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
More	analysis of structure and fold of the proenzyme, including the propeptide, interactions between the propeptide and the mature enzyme, modeling, overview	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
staphopain B	-	Staphylococcus aureus