Activating Compound | Comment | Organism | Structure |
---|---|---|---|
reducing thiol compounds | dependent on | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Staphylococcus aureus | |
alpha2-Macroglobulin | from human | Staphylococcus aureus | |
E-64 | stochiometrical and irreversible inhibition | Staphylococcus aureus | |
Hg2+ | - |
Staphylococcus aureus | |
additional information | no inhibition by human kininogens and cystatin C | Staphylococcus aureus | |
phosphorylated cystatin alpha | from rat skin | Staphylococcus aureus | |
T-Kininogen | from rat | Staphylococcus aureus | |
Zn2+ | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide | pH 8.0-8.8 | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | culture supernatant | Staphylococcus aureus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by changes in ionic strength by changing NaCl concentration | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Staphylococcus aureus | broad specificity | peptide proteolytically cleaved into fragments or single amino acids | - |
? | |
protein + H2O | Staphylococcus aureus | broad specificity | protein proteolytically cleaved into peptide fragments | - |
? | |
protein + H2O | Staphylococcus aureus | enzyme may play a role in growth regulation | protein proteolytically cleaved into peptide fragments | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
enzyme might be inducible by elastin in vivo | - |
Staphylococcus aureus | - |
V8 | - |
Staphylococcus aureus V8 | - |
V8 | - |
Purification (Comment) | Organism |
---|---|
native from culture supernatant | Staphylococcus aureus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Staphylococcus aureus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alcohol dehydrogenase + H2O | - |
Staphylococcus aureus | alcohol dehydrogenase proteolytically cleaved into peptide fragments | - |
? | |
alcohol dehydrogenase + H2O | - |
Staphylococcus aureus V8 | alcohol dehydrogenase proteolytically cleaved into peptide fragments | - |
? | |
benzyl-Tyr-OEt + H2O | - |
Staphylococcus aureus | ? | - |
? | |
casein + H2O | - |
Staphylococcus aureus | casein proteolytically cleaved into peptide fragments | - |
? | |
casein + H2O | - |
Staphylococcus aureus V8 | casein proteolytically cleaved into peptide fragments | - |
? | |
elastin + H2O | insoluble substrate | Staphylococcus aureus | elastin proteolytically cleaved into peptide fragments | - |
? | |
hemoglobin + H2O | - |
Staphylococcus aureus | hemoglobin proteolytically cleaved into peptide fragments | - |
? | |
hemoglobin + H2O | - |
Staphylococcus aureus V8 | hemoglobin proteolytically cleaved into peptide fragments | - |
? | |
N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide + H2O | - |
Staphylococcus aureus | N-benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline | - |
? | |
peptide + H2O | broad specificity | Staphylococcus aureus | amino acids | - |
? | |
peptide + H2O | broad specificity | Staphylococcus aureus V8 | amino acids | - |
? | |
peptide + H2O | broad specificity | Staphylococcus aureus | peptide proteolytically cleaved into fragments or single amino acids | - |
? | |
protein + H2O | broad specificity | Staphylococcus aureus | peptide fragments | - |
? | |
protein + H2O | broad specificity | Staphylococcus aureus V8 | peptide fragments | - |
? | |
protein + H2O | broad specificity | Staphylococcus aureus | protein proteolytically cleaved into peptide fragments | - |
? | |
protein + H2O | enzyme may play a role in growth regulation | Staphylococcus aureus | protein proteolytically cleaved into peptide fragments | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 13000, SDS-PAGE | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide | pH 8.0-8.8 | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 9.4 | Staphylococcus aureus |
6.5 | - |
substrate insoluble elastin | Staphylococcus aureus |
7.5 | - |
substrate benzyl-Tyr-OEt | Staphylococcus aureus |
8 | 8.8 | substrate casein or hemoglobin | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00052 | - |
E-64 | pH 8.0-8.8 | Staphylococcus aureus |